{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:03:17Z","timestamp":1788998597333,"version":"build-2803163510"},"reference-count":30,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2019,5,8]],"date-time":"2019-05-08T00:00:00Z","timestamp":1557273600000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Paroxysmal nocturnal hemoglobinuria (PNH) is a rare hematopoietic stem cell disorder resulting from the somatic mutation of the X\u2010linked phosphatidyl\u2010inositol glycan complementation Class A (PIG\u2010A) gene. Depending on the severity of the mutation in the PIG\u2010A gene, there is a partial or absolute inability to make glycosylphosphatidyl\u2010inositol (GPI)\u2010anchored proteins including complement\u2010defense structures such as CD55 and CD59 on RBCs and WBCs. Flow cytometric detection of PNH clones has become the gold standard and has played an increasingly important role in the diagnosis, monitoring, and clinical management of patients with PNH. Recently, a 4\u2010part set of Consensus Guidelines have been published by flow experts in the field to address the key assay\u2010specific considerations for the identification of PNH clones in RBC and WBC, how to report such data and a full validation document for the assays described. Below, we have summarized the most significant aspects of this International effort.<\/jats:p>","DOI":"10.1111\/ijlh.13011","type":"journal-article","created":{"date-parts":[[2019,5,9]],"date-time":"2019-05-09T05:29:15Z","timestamp":1557379755000},"page":"73-81","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Sensitive and accurate identification of PNH clones based on ICCS\/ESCCA PNH Consensus Guidelines\u2014A summary"],"prefix":"10.1111","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0237-3591","authenticated-orcid":false,"given":"Andrea J.","family":"Illingworth","sequence":"first","affiliation":[{"name":"Dahl\u2010Chase Diagnostic Services Bangor Maine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iuri","family":"Marinov","sequence":"additional","affiliation":[{"name":"Institute of Hematology and Blood Transfusion Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. Robert","family":"Sutherland","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Toronto Toronto Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,5,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2007.01.046"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hoc.2018.03.002"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejh.12299"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejh.12898"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20525"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.13853"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21023"},{"key":"e_1_2_9_9_1","first-page":"1","article-title":"High\u2010sensitivity detection of PNH red blood cells, red cell precursors and white blood cells","volume":"72","author":"Sutherland DR","year":"2015","journal-title":"Curr Protoc Cytom"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21610"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21139"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21480"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V36.2.145.145"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-03-010777"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21609"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cll.2017.07.007"},{"key":"e_1_2_9_17_1","unstructured":"SutherlandDR RichardsSJ OrtizF et\u00a0al. (2018)Addition of CD71 to high\u2010sensitivity CD235a\/CD59 PNH RBC assay allows the additional accurate delineation of PNH Type III PNH Type II and normal (Type I) nucleated RBCs(Abstract published at 33rdICCS meeting and course Portland OR September 28\u2010Cotober 2 2018)."},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21046"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21075"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21174"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21111"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/9781555818722.ch18"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12271"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21626"},{"key":"e_1_2_9_25_1","volume-title":"CLSI H52\u2010A2 Red Blood Cell Diagnostic Testing Using Flow Cytometry; Approved Guideline","author":"Davis BH","year":"2014"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20087"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21719"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-06-2485"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21615"},{"key":"e_1_2_9_30_1","unstructured":"IllingworthA OldakerT SutherlandDR et\u00a0al. (2018):Verification of PNH assay sensitivity through spiking experiment.https:\/\/www.cytometry.org\/web\/modules\/Module10.pdf"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21608"}],"container-title":["International Journal of Laboratory Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fijlh.13011","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.13011","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ijlh.13011","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.13011","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T17:41:50Z","timestamp":1694367710000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ijlh.13011"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5]]},"references-count":30,"journal-issue":{"issue":"S1","published-print":{"date-parts":[[2019,5]]}},"alternative-id":["10.1111\/ijlh.13011"],"URL":"https:\/\/doi.org\/10.1111\/ijlh.13011","archive":["Portico"],"relation":{},"ISSN":["1751-5521","1751-553X"],"issn-type":[{"value":"1751-5521","type":"print"},{"value":"1751-553X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,5]]},"assertion":[{"value":"2019-01-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-05-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}