{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T15:48:23Z","timestamp":1762876103758,"version":"3.40.5"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,5,3]],"date-time":"2019-05-03T00:00:00Z","timestamp":1556841600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Instrumentation Laboratory"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>HIT is caused by platelet\u2010activating IgG that recognize multimolecular PF4\/heparin complexes. HIT antibodies are generally detectable by PF4\u2010dependent enzyme immunoassay (EIA) and by platelet serotonin\u2010release assay (SRA) at the beginning of the HIT\u2010related platelet count fall. We determined whether an automated immunoassay for HIT, the latex immunoturbidimetric assay (LIA), also detects antibodies early during the course of HIT. The LIA was also used to evaluate a patient with putative SRA\u2010negative HIT.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We evaluated the timing and magnitude of LIA reactivity in serial plasma samples obtained from 19 SRA\u2010positive patients (17 with abnormal platelet count changes indicating HIT; two with subclinical seroconversion) and one putative SRA\u2010negative HIT patient, all obtained from patients who participated in a clinical trial of heparin thromboprophylaxis. We determined LIA status at the onset of the HIT\u2010related platelet count fall.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The LIA was positive in all 19 SRA\u2010positive patients (median value, 7.3\u00a0U\/mL [range, 1.2\u201035.5]; cutoff, 1.0\u00a0U\/mL); for all 13 evaluable patients for whom an informative plasma sample was available at (or shortly before) the onset of the HIT\u2010related platelet count fall, LIA reactivity was positive. Heterogeneity in seroconversion using the LIA was observed; some patients exhibited gradual increases in reactivity, whereas other patients showed rapid increase in reactivity over a few days. The single clinical trial patient who met clinical\u2010pathological criteria for \u201cSRA\u2010negative HIT\u201d tested LIA\u2010positive.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The LIA detects HIT antibodies at the beginning of the HIT\u2010associated platelet count fall. The LIA was also positive in a patient with SRA\u2010negative HIT.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.13031","type":"journal-article","created":{"date-parts":[[2019,5,3]],"date-time":"2019-05-03T15:29:10Z","timestamp":1556897350000},"page":"493-502","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Timeline of heparin\u2010induced thrombocytopenia seroconversion in serial plasma samples tested using an automated latex immunoturbidimetric assay"],"prefix":"10.1111","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8046-7588","authenticated-orcid":false,"given":"Theodore E.","family":"Warkentin","sequence":"first","affiliation":[{"name":"Department of Pathology and Molecular Medicine, Michael G. DeGroote School of Medicine McMaster University Hamilton Ontario Canada"},{"name":"Department of Medicine, Michael G. 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