{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T00:01:03Z","timestamp":1750204863725,"version":"3.40.5"},"reference-count":15,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,7,4]],"date-time":"2019-07-04T00:00:00Z","timestamp":1562198400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Chromogenic substrate assay (CSA) reagents Revohem<jats:sup>\u2122<\/jats:sup> FVIII and Revohem<jats:sup>\u2122<\/jats:sup> FIX are now available as in vitro diagnostic reagents for autoanalysers in Japan. In this study, we evaluated the performance of these reagents in the CS\u20105100 automated coagulation analyser.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We assessed within\u2010run and between\u2010day imprecision, on\u2010board stability and frozen\u2010storage stability of Revohem FVIII and FIX. Sensitivity to lupus anticoagulant (LA) was examined using LA\u2010positive patient plasma. Correlations were analysed using plasma samples from normal individuals and patients with haemophilia A (HA) or B (HB) or von Willebrand disease (VWD).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Imprecision was &lt;2% for Revohem FVIII and &lt;6.5% for Revohem FIX. On\u2010board storage of Revohem FVIII resulted in a &lt;10% decrease in FVIII levels from baseline at 24\u00a0hours, whereas Revohem FIX showed a &gt;10% decrease at 8\u00a0hours. Revohem FVIII showed good stability while frozen for 22\u00a0days. Although Revohem FIX showed degradation due to freeze\u2010thawing, a new calibration improved stability up to 22\u00a0days. Interference from LA was not observed with Revohem FVIII or FIX. The FVIII CSA\u2010CSA correlation was excellent in normal (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.9924), HA (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.9945) and VWD (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.9914). The FVIII CSA\u2010OSA correlation was good in normal (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.8468) and <jats:italic>excellent<\/jats:italic> in HA (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.975) and VWD (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.9936). The FIX CSA\u2010OSA correlation was fair in normal (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.4791) and excellent in HB (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.9501).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Revohem FVIII and FIX both showed excellent performance in the CS\u20105100 analyser. These reagents could be useful in routine laboratory testing for diagnosing and treating haemophilia.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.13083","type":"journal-article","created":{"date-parts":[[2019,7,4]],"date-time":"2019-07-04T17:26:05Z","timestamp":1562261165000},"page":"664-670","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Performance evaluation of Revohem<sup>\u2122<\/sup> FVIII chromogenic and Revohem<sup>\u2122<\/sup> FIX chromogenic in the CS\u20105100 autoanalyser"],"prefix":"10.1111","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7194-3183","authenticated-orcid":false,"given":"Atsuo","family":"Suzuki","sequence":"first","affiliation":[{"name":"Department of Medical Technique Nagoya University Hospital Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuaki","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine Nagoya University Hospital Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Kanematsu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Nagoya University Hospital Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sho","family":"Shinohara","sequence":"additional","affiliation":[{"name":"Sysmex Corporation Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuo","family":"Arai","sequence":"additional","affiliation":[{"name":"Sysmex Corporation Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryosuke","family":"Kikuchi","sequence":"additional","affiliation":[{"name":"Department of Medical Technique Nagoya University Hospital Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tadashi","family":"Matsushita","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine Nagoya University Hospital Nagoya Japan"},{"name":"Department of Laboratory Medicine Nagoya University Hospital Nagoya 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