{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:52:20Z","timestamp":1787014340641,"version":"build-2736575974"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,11,26]],"date-time":"2019-11-26T00:00:00Z","timestamp":1574726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Early detection of endothelial graft\u2010vs\u2010host disease (\n                      <jats:styled-content style=\"fixed-case\">GVHD<\/jats:styled-content>\n                      ) after hematopoietic stem cell transplantation (\n                      <jats:styled-content style=\"fixed-case\">HSCT<\/jats:styled-content>\n                      ) might help protect the patient from the late and severe complications of transplant\u2010associated thrombotic microangiopathy (\n                      <jats:styled-content style=\"fixed-case\">TA<\/jats:styled-content>\n                      \u2010\n                      <jats:styled-content style=\"fixed-case\">TMA<\/jats:styled-content>\n                      ). Appearance of schistocytes in peripheral blood is one of the cardinal diagnostic features of\n                      <jats:styled-content style=\"fixed-case\">TA<\/jats:styled-content>\n                      \u2010\n                      <jats:styled-content style=\"fixed-case\">TMA<\/jats:styled-content>\n                      . Our aim was to test the diagnostic accuracy and objectiveness of digital microscopy with respect to the recognition and quantification of schistocytes in the setting of endothelial\n                      <jats:styled-content style=\"fixed-case\">GVHD<\/jats:styled-content>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Peripheral blood smears from 127 allogeneic and 63 autologous\n                      <jats:styled-content style=\"fixed-case\">HSCT<\/jats:styled-content>\n                      patients (January 2016\u2010June 2017) were retrospectively examined before, 1 month and at 2\u20103 months after transplantation using digital microscopy. Peripheral blood smears from 31 healthy blood donors were also analyzed as the control group.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Assessment by digital microscopy showed that schistocytes are significantly increased after 3 months from the allogeneic (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a0.001) or the autologous\n                      <jats:styled-content style=\"fixed-case\">HSCT<\/jats:styled-content>\n                      (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a0.001) compared to the control group. Significantly higher schistocyte counts were found in patients with acute\n                      <jats:styled-content style=\"fixed-case\">GVHD<\/jats:styled-content>\n                      (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a0.05) and in patients with\n                      <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>\n                      mismatch (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a0.005). Patients in the upper quartile of the schistocyte counts had significantly more frequently acute\n                      <jats:styled-content style=\"fixed-case\">GVHD<\/jats:styled-content>\n                      (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a0.024) or\n                      <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>\n                      mismatch (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a0.035).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Schistocytes can be reliably counted by means of a digital microscopy system and are significantly higher in patients with acute\n                      <jats:styled-content style=\"fixed-case\">GVHD<\/jats:styled-content>\n                      and\n                      <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>\n                      mismatch. Inversely, patients with the highest numbers of schistocytes are more frequently affected by\n                      <jats:styled-content style=\"fixed-case\">aGVHD<\/jats:styled-content>\n                      , implying that high schistocyte counts after\n                      <jats:styled-content style=\"fixed-case\">HSCT<\/jats:styled-content>\n                      might be a surrogate marker for this complication.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ijlh.13130","type":"journal-article","created":{"date-parts":[[2019,11,26]],"date-time":"2019-11-26T11:34:00Z","timestamp":1574768040000},"page":"145-151","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Peripheral blood schistocytes in the acute phase after allogeneic or autologous stem cell transplantation assessed by digital 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