{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:16:51Z","timestamp":1747185411000,"version":"3.40.5"},"reference-count":12,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,12,10]],"date-time":"2019-12-10T00:00:00Z","timestamp":1575936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Shinshu Medical Promotion Association"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Detection of lupus anticoagulant (LA), an antiphospholipid (aPL) antibody, in a clotting time test is an important finding for diagnosis of antiphospholipid syndrome (APS). However, confirmation of LA requires several different testing procedures, some of which can be difficult and require time. We report here a simple and highly specific method for detecting LA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>We examined 66 plasma samples obtained from LA\u2010positive (LA) and 75 from LA\u2010negative (non\u2010LA) subjects, which included patients with acquired hemophilia and coagulation disorders, as well as from 43 healthy volunteer samples as normal controls. Activated partial thromboplastin time (APTT) was determined by adding 20\u00a0mmol of CaCl<jats:sub>2<\/jats:sub> (Ca\u2010APTT) or 25\u00a0mmol of a mixture of Mg and Ca (Mg\u2010APTT). The ratio of Mg\u2010APTT\/Ca\u2010APTT was then calculated and used as the Mg\/Ca Index.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The Mg\/Ca Index value for the LA group was significantly lower than that for the non\u2010LA and normal control groups (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.0001). When the cutoff value of the Mg\/Ca Index was less than 1.00, the sensitivity of LA determination using the Mg\u2010APTT assay was 80.3%, while specificity was 100%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings indicate that the present Mg\u2010APTT assay is a simple yet highly specific method for LA detection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.13134","type":"journal-article","created":{"date-parts":[[2019,12,10]],"date-time":"2019-12-10T15:42:09Z","timestamp":1575992529000},"page":"46-51","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Magnesium\u2010dependent activated partial thromboplastin time assay\u2014Simple method for lupus anticoagulant detection"],"prefix":"10.1111","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6952-4908","authenticated-orcid":false,"given":"Takayoshi","family":"Tokutake","sequence":"first","affiliation":[{"name":"Department of Transfusion Nagano Red Cross Hospital Nagano Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Ieko","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine School of Dentistry Health Sciences University of Hokkaido Ishikari\u2010Tobetsu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sumiyoshi","family":"Naito","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Hospital of Health Sciences University of Hokkaido Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mika","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Health Sciences University of Hokkaido Ishikari\u2010Tobetsu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hisami","family":"Baba","sequence":"additional","affiliation":[{"name":"Department of Transfusion Nagano Red Cross Hospital Nagano Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hikaru","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Transfusion Nagano Red Cross Hospital Nagano Japan"},{"name":"Department of Hematology Nagano Red Cross Hospital Nagano Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fumihiro","family":"Ishida","sequence":"additional","affiliation":[{"name":"Department of Biomedical Laboratory Sciences School of Health Sciences Shinshu University School of Medicine Matsumoto 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