{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T21:48:57Z","timestamp":1780436937336,"version":"3.54.1"},"reference-count":19,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,3,23]],"date-time":"2020-03-23T00:00:00Z","timestamp":1584921600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81170472"],"award-info":[{"award-number":["81170472"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Hemorrhage and infection are two main causes of death in patients with myelodysplastic syndromes (MDS), and it is becoming increasingly clear that platelet dysfunction can also affect the process of hemostasis and anti\u2010infection. The aim of this study was to evaluate activation function and immune\u2010related function of platelets in MDS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We included 29 MDS patients and divided them into different subgroups (low\u2010risk group and high\u2010risk group; untreated group and treated group; pretransfusion group and post\u2010transfusion group) according to IPSS\u2010R score, hypomethylating agents (HMAs) therapy, and platelet transfusion history. Platelet light scatter properties, expression of CD41a, activation\u2010associated phenotypes (CD62p and CD63), and immune\u2010associated phenotypes (CD154 and TLR4) were detected by multiparameter flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Expression of CD41a was decreased (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05), and no difference was found in platelet light scatter properties between MDS patients and healthy subjects (<jats:italic>P<\/jats:italic>\u00a0&gt;\u00a0.05). Significantly decreased expression frequency and intensity of activation phenotype CD63 were found in patients with MDS (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05). Low\u2010risk MDS showed lower expression frequency while high\u2010risk MDS showed reduced mean fluorescence intensity (MFI) of CD63. Decreased expression of CD154 and TLR4 was found in MDS patients (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05) which was significantly elevated after HMAs therapy (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05). Particularly, MFI of CD154 and TLR4 reduced in high\u2010risk MDS patients (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Myelodysplastic syndromes patients displayed defective expression of both activation\u2010 and immune\u2010associated platelet phenotypes, with differential mechanisms between low\u2010risk and high\u2010risk groups regarding phenotype alterations. The findings confirmed impaired platelet phenotypes in MDS which may assist in the diagnosis and identification of MDS patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.13191","type":"journal-article","created":{"date-parts":[[2020,3,23]],"date-time":"2020-03-23T08:40:33Z","timestamp":1584952833000},"page":"371-379","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Abnormal platelet phenotypes in patients with myelodysplastic syndromes"],"prefix":"10.1111","volume":"42","author":[{"given":"Jiaxi","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Hematology General Hospital Tianjin Medical University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyue","family":"Niu","sequence":"additional","affiliation":[{"name":"Department of Hematology General Hospital Tianjin Medical University Tianjin 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