{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T04:18:54Z","timestamp":1781756334370,"version":"3.54.5"},"reference-count":11,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,5,28]],"date-time":"2020-05-28T00:00:00Z","timestamp":1590624000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"SGPGIMS","award":["A-15-PGI\/IMP\/75\/2017"],"award-info":[{"award-number":["A-15-PGI\/IMP\/75\/2017"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>About 50 different <jats:italic>CALR<\/jats:italic> frameshift mutations have been identified in BCR\u2010ABL1 negative MPN, all leading to the development of common new protein C terminus. Antibody targeting this terminal epitope can be useful to identify this driver mutation using immunohistochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p><jats:italic>CALR<\/jats:italic> mutation analysis was carried out in 51 <jats:italic>JAK2V617F<\/jats:italic> negative cases, PMF (n\u00a0=\u00a022) and ET (n\u00a0=\u00a029). PCR followed by fragment analysis was performed for molecular detection of <jats:italic>CALR<\/jats:italic> mutation. Bone marrow biopsy specimens of corresponding patients were subjected to IHC using mutation specific antibody CAL2. Staining pattern and intensity were observed. Staining of &lt;2% of background nonmegakaryocytic (non\u2010 MK) cells were regarded as Pattern A, while staining of more than 2% of background nonmegakaryocytic (non\u2010MK) was regarded as pattern B.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>CALR<\/jats:italic> mutation was noted in 40.9% (9\/22) and 41.4% (12\/29) of <jats:italic>JAK2V617F<\/jats:italic> negative PMF and ET, respectively. All <jats:italic>CALR<\/jats:italic> mutated cases, irrespective of the mutation type, showed a positive IHC staining in the megakaryocytes with moderate to bright intensity. All <jats:italic>CALR<\/jats:italic> wild\u2010type cases were negative on IHC. (Concordance rate\u2010 100%). Pattern A was noted in 40% cases, while pattern B was noted in 60% cases. Pattern A staining had significantly higher chances of having type 1 mutation as compared to pattern B. In contrast, pattern B had a nonsignificant trend toward higher bone marrow cellularity and marrow fibrosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>CAL2 IHC detects all types of <jats:italic>CALR<\/jats:italic> mutation. This can act as a sensitive, specific, rapid, and cost\u2010effective screening test for <jats:italic>CALR<\/jats:italic> mutation analysis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.13242","type":"journal-article","created":{"date-parts":[[2020,5,28]],"date-time":"2020-05-28T11:59:20Z","timestamp":1590667160000},"page":"604-611","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Mutant specific anti calreticulin antibody (CAL2) immunohistochemistry as a screening test for calreticulin (<i>CALR<\/i>) mutation testing"],"prefix":"10.1111","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8285-2092","authenticated-orcid":false,"given":"Khaliqur","family":"Rahman","sequence":"first","affiliation":[{"name":"Department of Hematology SGPGI Lucknow Uttar Pradesh 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