{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T16:04:01Z","timestamp":1790352241441,"version":"4.1.0"},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,10,23]],"date-time":"2020-10-23T00:00:00Z","timestamp":1603411200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Thrombotic thrombocytopenic purpura (TTP) is a life\u2010threatening disorder characterized by severe ADAMTS13 deficiency. The acquired form is associated with autoantibodies directed against ADAMTS13. Both noninhibitory and inhibitory autoantibodies can be detected by ELISA assay, while only inhibitory autoantibodies are detected by Bethesda assay. Due to its short TAT and good performance, chemiluminescence (CliA) ADAMTS13 activity (HemosIL Acustar) has proven to be a good choice in the diagnosis of TTP in emergency settings. Aim of this study was to analyse the performance of the CliA ADAMTS13 activity assay in detecting inhibitory ADAMTS13 antibodies using the Bethesda assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A method comparison study was performed on 69 stored samples: 11 acute TTPs, 38 TTP follow\u2010ups, 5 TTP relapses, 1 congenital TTP, 10 HUS, 4 suspected TTPs. We retrieved the results of tests previously run in ELISA for both activity and autoantibodies. At the same time, we reran new tests including ELISA and CliA activity, ELISA autoantibodies, and ELISA and CliA Bethesda assays on thawed frozen samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Very good correlation was observed between ELISA and CliA activity assay results (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.96) and between archived ELISA and CliA activity results (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.93). Agreement between the anti\u2010ADAMTS13 assays ranged from good (<jats:italic>k<\/jats:italic>\u00a0=\u00a00.63) to very good (<jats:italic>k<\/jats:italic>\u00a0=\u00a00.92).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>CliA and ELISA Bethesda assays showed very good agreement with samples run at the same time using ELISA ADAMTS13\u2010autoantibody assay. Albeit more expensive, the CliA Bethesda assay identified inhibitory anti\u2010ADAMTS13 within almost the same TAT as ELISA, but with better automation and limited operator involvement.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.13372","type":"journal-article","created":{"date-parts":[[2020,10,23]],"date-time":"2020-10-23T17:18:04Z","timestamp":1603473484000},"page":"298-304","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Inhibitory anti ADAMTS13 antibodies with a new rapid fully automated CLiA assay"],"prefix":"10.1111","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5713-2069","authenticated-orcid":false,"given":"Barbara","family":"Montaruli","sequence":"first","affiliation":[{"name":"S.C. Laboratory Analysis AO Ordine Mauriziano  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Novelli","sequence":"additional","affiliation":[{"name":"Blood Transfusion Center Legnano General Hospital  Legnano Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Solfietti","sequence":"additional","affiliation":[{"name":"Department of Clinical and Biological Sciences, and SCDU Nephrology and Dialysis Center for Research of Immunopathology and Rare Diseases Coordinating Center of Piemonte and Valle d'Aosta Network for Rare Diseases S. Giovanni Bosco Hospital  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Valpreda","sequence":"additional","affiliation":[{"name":"Laboratory of the Regional Reference Center for Adult Thrombotic and Hemorrhagic Diseases A.O.U. Citt\u00e0 della Salute e della Scienza  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Bazzan","sequence":"additional","affiliation":[{"name":"Department of Clinical and Biological Sciences, and SCDU Nephrology and Dialysis Center for Research of Immunopathology and Rare Diseases Coordinating Center of Piemonte and Valle d'Aosta Network for Rare Diseases S. Giovanni Bosco Hospital  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivo","family":"Luigi Andrea Beverina","sequence":"additional","affiliation":[{"name":"Blood Transfusion Center Legnano General Hospital  Legnano Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Brando","sequence":"additional","affiliation":[{"name":"Blood Transfusion Center Legnano General Hospital  Legnano Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dario","family":"Roccatello","sequence":"additional","affiliation":[{"name":"Department of Clinical and Biological Sciences, and SCDU Nephrology and Dialysis Center for Research of Immunopathology and Rare Diseases Coordinating Center of Piemonte and Valle d'Aosta Network for Rare Diseases S. Giovanni Bosco Hospital  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Domenico","family":"Cosseddu","sequence":"additional","affiliation":[{"name":"S.C. Laboratory Analysis AO Ordine Mauriziano  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,10,23]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2012.09167.x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-10-709857"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.13571"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2018-02-791533"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-02-006064"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2009.03568.x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2006.02025.x"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199811263392203"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2004.05217.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-05-1616"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1653759"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13359"},{"issue":"1","key":"e_1_2_9_14_1","first-page":"1297","article-title":"Highly sensitive fully automated chemiluminescent immunoassay for rapid quantification of ADAMTS13 activity","volume":"1","author":"Mirabet M","year":"2017","journal-title":"Res Pract Thromb Haemost"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0039-1696718"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1641151"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.15219"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1672187"},{"key":"e_1_2_9_19_1","first-page":"PO39","article-title":"ADAMTS13 antibodies assessment with the Hemosil Acustar ADAMTS13 Activity Assay. Joint 10th BIC and 3rd Inhibitors in Hemophilia International Conference Genoa Italy 6\u20138 Sept 2019","volume":"23","author":"Valsecchi C","year":"2019","journal-title":"Haematologica"},{"key":"e_1_2_9_20_1","volume-title":"Transport and Processing of Blood Specimens for Testing Plasma\u2010Based Coagulation Assays and Molecular Hemostasis Assays. Approved Guidelines\u2010Fifth Edition","author":"Adcock DM","year":"2008"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.14440"}],"container-title":["International Journal of Laboratory Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.13372","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ijlh.13372","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.13372","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T14:31:07Z","timestamp":1693578667000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ijlh.13372"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,23]]},"references-count":20,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021,4]]}},"alternative-id":["10.1111\/ijlh.13372"],"URL":"https:\/\/doi.org\/10.1111\/ijlh.13372","archive":["Portico"],"relation":{},"ISSN":["1751-5521","1751-553X"],"issn-type":[{"value":"1751-5521","type":"print"},{"value":"1751-553X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,23]]},"assertion":[{"value":"2020-06-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}