{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T16:54:45Z","timestamp":1788195285331,"version":"build-2803163510"},"reference-count":16,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,10,28]],"date-time":"2020-10-28T00:00:00Z","timestamp":1603843200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81930005"],"award-info":[{"award-number":["81930005"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81871263"],"award-info":[{"award-number":["81871263"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81700177"],"award-info":[{"award-number":["81700177"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Anti\u2010CD19 chimeric antigen receptor (CAR) \u2010T cells, which recognize and kill both B lymphoblasts and normal B cells, result in B cell aplasia and humoral immunodeficiency. However, there were only a few detailed reports on the profile of immune reconstitution after anti\u2010CD19 CAR\u2010T cell therapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Thirty nine patients with relapsed or refractory (R\/R) B cell acute lymphoblastic leukemia (ALL) receiving anti\u2010CD19 CAR\u2010T cell therapy were enrolled. Subjects died, relapsed, received other treatment, or lost to follow\u2010up within 60\u00a0days post\u2010infusion were excluded. 21 patients were finally selected. Laboratory and clinical data were collected for analysis of immune reconstitution.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CD8+ cells were the first to recover with a median time on day 21(7\u201087), followed by CD16\/CD56+ cells on day 28(14\u201087), and finally CD4+ cells with only 5(23.81%) patients recovered within 60\u00a0days post\u2010infusion. CD4\/CD8 ratio was inverted, sustaining for at least 1\u00a0year. B cell aplasia occurred in all patients and CD19+ cells returned to normal on a median time of day 79(41\u2010118). All patients developed hypogammaglobulinemia with a median onset time of 2\u00a0weeks post\u2010infusion. IgG recovered in 6 patients with a median time on day 184(89\u2010346). IgM recovered on days 212, 242, and 346 in 3 patients. IgA recovered most slowly and remained low &gt;1\u00a0year postinfusion. A total of 9 infections occurred in 6(28.57%) patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our data showed prolonged reconstitution of immune function, especially humoral immunity, in R\/R B cell ALL patients receiving anti\u2010CD19 CAR\u2010T cell therapy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.13375","type":"journal-article","created":{"date-parts":[[2020,10,28]],"date-time":"2020-10-28T14:09:11Z","timestamp":1603894151000},"page":"250-258","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Kinetics of immune reconstitution after anti\u2010CD19 chimeric antigen receptor T cell therapy in relapsed or refractory acute lymphoblastic leukemia patients"],"prefix":"10.1111","volume":"43","author":[{"given":"Ying","family":"Wang","sequence":"first","affiliation":[{"name":"Blood Diseases Institute Xuzhou Medical University Xuzhou China"},{"name":"Department of Hematology The Affiliated Hospital of Xuzhou Medical University Xuzhou China"},{"name":"Jiangsu Key Laboratory of Bone Marrow Stem Cells Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hujun","family":"Li","sequence":"additional","affiliation":[{"name":"Blood Diseases Institute Xuzhou Medical University Xuzhou China"},{"name":"Department of Hematology The Affiliated Hospital of Xuzhou Medical University Xuzhou China"},{"name":"Jiangsu Key Laboratory of Bone Marrow Stem Cells Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuguang","family":"Song","sequence":"additional","affiliation":[{"name":"Blood Diseases Institute Xuzhou Medical University Xuzhou China"},{"name":"Department of Hematology The Affiliated Hospital of Xuzhou Medical University Xuzhou China"},{"name":"Jiangsu Key Laboratory of Bone Marrow Stem Cells Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunming","family":"Qi","sequence":"additional","affiliation":[{"name":"Blood Diseases Institute Xuzhou Medical University Xuzhou China"},{"name":"Department of Hematology The Affiliated Hospital of Xuzhou Medical University Xuzhou China"},{"name":"Jiangsu Key Laboratory of Bone Marrow Stem Cells Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai","family":"Cheng","sequence":"additional","affiliation":[{"name":"Blood Diseases Institute Xuzhou Medical University Xuzhou China"},{"name":"Department of Hematology The Affiliated Hospital of Xuzhou Medical University Xuzhou China"},{"name":"Jiangsu Key Laboratory of Bone Marrow Stem Cells Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiang","family":"Cao","sequence":"additional","affiliation":[{"name":"Blood Diseases Institute Xuzhou Medical University Xuzhou China"},{"name":"Department of Hematology The Affiliated Hospital of Xuzhou Medical University Xuzhou China"},{"name":"Jiangsu Key Laboratory of Bone Marrow Stem Cells Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Shi","sequence":"additional","affiliation":[{"name":"Cancer Institute Xuzhou Medical University Xuzhou China"},{"name":"Center of Clinical Oncology The Affiliated Hospital of Xuzhou Medical University Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiling","family":"Yan","sequence":"additional","affiliation":[{"name":"Blood Diseases Institute Xuzhou Medical University Xuzhou China"},{"name":"Department of Hematology The Affiliated Hospital of Xuzhou Medical University Xuzhou China"},{"name":"Jiangsu Key Laboratory of Bone Marrow Stem Cells Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangjun","family":"Jing","sequence":"additional","affiliation":[{"name":"iCARTAB biomedical co. 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