{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T15:00:14Z","timestamp":1789052414988,"version":"build-2803163510"},"reference-count":18,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,12,2]],"date-time":"2020-12-02T00:00:00Z","timestamp":1606867200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Thrombotic thrombocytopenic purpura (TTP) is a rare life\u2010threatening thrombotic microangiopathy (TMA) characterized by the severe deficiency of ADAMTS13 activity (&lt;10%). Rapid ADAMTS13 testing is crucial for early diagnosis and optimal management of TTP patients and other TMAs. The objective of this study was to retrospectively evaluate the performance of the recently commercialized HemosIL Acustar\n                      <jats:sup>\u00ae<\/jats:sup>\n                      ADAMTS13 activity chemiluminescent immunoassay (Instrumentation Laboratory, Bedford, Massachusetts, United States) in a multicentric study between Spain and Portugal.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A comparison method was performed to compare HemosIL Acustar\n                      <jats:sup>\u00ae<\/jats:sup>\n                      with an in\u2010house FRETS\u2010VWF73 assay and two commercial ELISA assays: the TECHNOZYM\n                      <jats:sup>\u00ae<\/jats:sup>\n                      ADAMTS13 Activity (Technoclone GmbH, Vienna, Austria) and the DG\u2010EIA ADAMTS\u201013 Activity (Diagnostic Grifols, SA, Barcelona, Spain). A set of 241 frozen plasma samples with known ADAMST13 levels was used. Agreement between methods was assessed with focus on two cut\u2010off ADAMTS13 activity values: &lt;10% (the clinical accepted cut\u2010off value to confirm TTP diagnosis) and\u00a0&lt;5%.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      HemosIL AcuStar\n                      <jats:sup>\u00ae<\/jats:sup>\n                      showed high agreement with the other methods in correctly classify patients with ADAMTS13 values below 10% (Kappa\u00a0=\u00a00.89) and even below 5% (Kappa\u00a0=\u00a00.94) with no false negatives and few false positives (5.40%; 95% CI: 2.20 to 8.60%). However, it also tended to underestimate ADAMTS13 levels, especially for the high assay range values (&gt;40%) (absolute mean bias of 8.40% (95% CI: 6.53 to 10.42%)) when compared to other assays.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      HemosIL AcuStar\n                      <jats:sup>\u00ae<\/jats:sup>\n                      is highly sensitive to detect ADAMTS13 values below 10% and 5%. A large prospective validation study is needed to corroborate its utility in clinical practice.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ijlh.13414","type":"journal-article","created":{"date-parts":[[2020,12,2]],"date-time":"2020-12-02T17:38:25Z","timestamp":1606930705000},"page":"485-493","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Multicentric evaluation of the new HemosIL Acustar\n                    <sup>\u00ae<\/sup>\n                    chemiluminescence ADAMTS13 activity assay"],"prefix":"10.1111","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5442-4886","authenticated-orcid":false,"given":"Cristina","family":"Pascual","sequence":"first","affiliation":[{"name":"University Hospital Gregorio Mara\u00f1\u00f3n  Madrid Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1on  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge M.","family":"Nieto","sequence":"additional","affiliation":[{"name":"University Hospital Cl\u00ednico San Carlos  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa","family":"Fidalgo","sequence":"additional","affiliation":[{"name":"Hospital and Universitary Center of Coimbra  Coimbra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"In\u00e9s G\u00f3mez","family":"Segu\u00ed","sequence":"additional","affiliation":[{"name":"La Fe University and Polytechnic Hospital  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maribel","family":"D\u00edaz\u2010Ricart","sequence":"additional","affiliation":[{"name":"Hematopathology, Pathology Department, Centre de Diagnostic Biomedic (CDB), Institut d'Investigacions Biom\u00e8diques August Pi i Sunyer (IDIBAPS), Hospital Cl\u00ednic de Barcelona University of Barcelona  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta Fern\u00e1ndez","family":"Docampo","sequence":"additional","affiliation":[{"name":"University Hospital A Coru\u00f1a  A Coru\u00f1a Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julio","family":"del Rio","sequence":"additional","affiliation":[{"name":"University Hospital of Orense  Orense Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramon","family":"Salinas","sequence":"additional","affiliation":[{"name":"Hospital del Sagrat Cor Universitat Internacional de Catalunya Banc de Sang i Teixits de Catalunya  Barcelona 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