{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T14:29:54Z","timestamp":1753885794350,"version":"3.40.5"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,12,12]],"date-time":"2020-12-12T00:00:00Z","timestamp":1607731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008982","name":"Qatar National Research Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008982","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007833","name":"Hamad Medical Corporation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007833","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Lymphoid enhancer\u2010binding factor 1 (<jats:italic>LEF\u20101<\/jats:italic>) overexpression has been recently remarkably reported in chronic lymphocytic leukemia\/small lymphocytic lymphoma (CLL\/SLL) and has shown utility in distinguishing CLL\/SLL from other B\u2010cell lymphomas. CLL has a well\u2010defined immunophenotype, yet, some cases of CLL demonstrate atypical morphology\/ phenotype reflected by low Matutes score (atypical CLL). Till date, <jats:italic>LEF1<\/jats:italic> expression has not been systematically studied in cases of CLL with atypical features.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study, <jats:italic>LEF\u20101<\/jats:italic> expression was assessed by two different techniques, (immunohistochemistry and flow cytometry), to investigate the expression profile of <jats:italic>LEF\u20101<\/jats:italic> in cases of CLL\/SLL, in comparison with other low\u2010grade B\u2010lymphomas and CLL with atypical features, including atypical immunophenotype and CLL with increased prolymphocytes or morphologically atypical cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that <jats:italic>LEF\u20101<\/jats:italic> expression is downregulated in CLL with atypical immunophenotype\/features compared to classic CLL; Chi\u2010Square <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.0001. The ratio for <jats:italic>LEF\u20101<\/jats:italic> expression in malignant B\u2010cells\/NK (by flow cytometry) in CLL\/SLL with classic immunophenotype was higher than atypical CLL and is significantly higher in other small B\u2010cell lymphomas (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a0.01). Absence of <jats:italic>LEF\u20101<\/jats:italic> expression in CLL\/SLL is correlated (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05) with downregulation of CD5, CD23, CD200, expression of FMC7, brighter expression of CD79b, brighter expression of surface light chain, increased prolymphocytes and lower Matutes score.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>As downregulation of <jats:italic>LEF\u20101<\/jats:italic> expression is well correlated with atypical CLL, we suggest adding <jats:italic>LEF\u20101<\/jats:italic> to Matutes score as a beneficial marker to differentiate classic from atypical CLL <jats:italic>LEF\u20101<\/jats:italic> could also serve as a potential prognostic indicator for CLL clinical course.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.13420","type":"journal-article","created":{"date-parts":[[2020,12,12]],"date-time":"2020-12-12T15:54:08Z","timestamp":1607788448000},"page":"515-525","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Downregulation of Lymphoid enhancer\u2010binding factor 1 (<i>LEF\u20101)<\/i> expression (by immunohistochemistry and\/ flow cytometry) in chronic Lymphocytic Leukemia with atypical immunophenotypic and cytologic features"],"prefix":"10.1111","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0624-3973","authenticated-orcid":false,"given":"Dina S.","family":"Soliman","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Pathology National Center for Cancer Care and Research Hamad Medical Corporation  Doha Qatar"},{"name":"Weill Cornell Medicine \u2013 Qatar  Doha Qatar"},{"name":"Department of Clinical Pathology National Cancer Institute  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Einas","family":"Al\u2010Kuwari","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Hamad Medical Corporation  Doha Qatar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kodappully S","family":"Siveen","sequence":"additional","affiliation":[{"name":"Flow Cytometry Core Facility interim Translational Research Institute Hamad Medical Corporation  Doha Qatar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajaa","family":"Al\u2010Abdulla","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Hamad Medical Corporation  Doha Qatar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prem","family":"Chandra","sequence":"additional","affiliation":[{"name":"Medical Research Center Academic Health Systems Hamad Medical Corporation  Doha Qatar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed","family":"Yassin","sequence":"additional","affiliation":[{"name":"Department of Hematology and Medical Oncology National Center for Cancer Care and Research Hamad Medical Corporation  Doha Qatar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdulqader","family":"Nashwan","sequence":"additional","affiliation":[{"name":"Nursing Department Hazm Mebaireek General Hospital Hamad Medical Corporation  Doha Qatar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feryal A","family":"Hilmi","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology National Center for Cancer Care and Research Hamad Medical Corporation  Doha Qatar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruba Y","family":"Taha","sequence":"additional","affiliation":[{"name":"Department of Hematology and Medical Oncology National Center for Cancer Care and Research Hamad Medical Corporation  Doha Qatar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zafar","family":"Nawaz","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Hamad Medical Corporation  Doha Qatar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Halima","family":"El\u2010Omri","sequence":"additional","affiliation":[{"name":"Department of Hematology and Medical Oncology National Center for Cancer Care and Research Hamad Medical Corporation  Doha Qatar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jericha M","family":"Mateo","sequence":"additional","affiliation":[{"name":"Flow Cytometry Core Facility interim Translational Research Institute Hamad Medical Corporation  Doha Qatar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmad","family":"Al\u2010Sabbagh","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology National Center for Cancer Care and Research Hamad Medical Corporation  Doha Qatar"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,12,12]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-06-093906"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/108.4.378"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1996.d01-1478.x"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPPAG4VR4IPGHZ"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-02-269878"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s000180050449"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(99)80031-3"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg1427"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2172-7-13"},{"key":"e_1_2_11_11_1","first-page":"346","article-title":"Identification of a global gene expression signature of B\u2010chronic lymphocytic leukemia","volume":"1","author":"Jelinek DF","year":"2003","journal-title":"Mol Cancer Res"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200323840"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2014-0058-RA"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2011.103"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPT32JDFIGHFHJ"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1997.402686.x"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2017.186684"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.42.6.567"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.beha.2010.01.001"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12930"},{"volume-title":"WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues","year":"2017","author":"Swerdlow SH","key":"e_1_2_11_21_1"},{"volume-title":"Pathology and Genetics of Tumours of Haematopoietic and Lymphoid Tissues","year":"2001","author":"Jaffe ES","key":"e_1_2_11_22_1"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21128"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12167"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2012.12.013"},{"key":"e_1_2_11_26_1","first-page":"1","article-title":"Alexandar Tzankov. LEF1: a highly specific marker for the diagnosis of chronic lymphocytic B cell leukaemia\/small lymphocytic B cell lymphoma","author":"Menter T","year":"2015","journal-title":"J Clin Pathol"},{"key":"e_1_2_11_27_1","first-page":"292","article-title":"Diagnostic utility of lymphoid enhancer binding factor 1 immunohistochemistry in small B\u2010cell lymphomas","volume":"147","author":"Menter T","year":"2017","journal-title":"Am J Clin Pathol"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200012283432602"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-11-312421"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64287-3"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0125429"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.12659\/AJCR.921131"}],"container-title":["International Journal of Laboratory Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.13420","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ijlh.13420","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.13420","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T04:11:47Z","timestamp":1693627907000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ijlh.13420"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,12]]},"references-count":31,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,6]]}},"alternative-id":["10.1111\/ijlh.13420"],"URL":"https:\/\/doi.org\/10.1111\/ijlh.13420","archive":["Portico"],"relation":{},"ISSN":["1751-5521","1751-553X"],"issn-type":[{"type":"print","value":"1751-5521"},{"type":"electronic","value":"1751-553X"}],"subject":[],"published":{"date-parts":[[2020,12,12]]},"assertion":[{"value":"2020-07-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}