{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T01:04:33Z","timestamp":1770339873584,"version":"3.49.0"},"reference-count":42,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,12,12]],"date-time":"2020-12-12T00:00:00Z","timestamp":1607731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Activated protein C (APC) resistance is a major risk factor of venous thrombosis which may be acquired by hormonal therapy or other causes. The FibWave, a sensitive global clot\u2010based assay design to analyze the coagulation kinetics in plasma, may be a good candidate to assess this prothrombotic state. This study aims to assess the suitability of the FibWave to differentiate the coagulation kinetics of women on oral contraceptives.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Fifty\u2010four healthy volunteers were divided into 5 groups: men [n\u00a0=\u00a013], women not using hormonal contraception [n\u00a0=\u00a012], women using second [n\u00a0=\u00a012] or third generation [n\u00a0=\u00a012] combined oral contraceptives, and women using progestin only contraceptive [n\u00a0=\u00a05]. Patients with coagulation abnormalities were also assessed [n\u00a0=\u00a08]. The APC resistance was assessed on the FibWave using exogenous APC or Protac, and on the Calibrated Automated Thrombogram using the ETP\u2010based APC resistance assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Either in presence or in absence of APC or Protac, the FibWave was able to detect a hypercoagulable state in plasma samples. All combined oral contraceptives showed a lower FW\u2010Max<jats:sub>1<\/jats:sub>, FW\u2010Max<jats:sub>2,<\/jats:sub> and FW\u2010Min<jats:sub>2<\/jats:sub> percentage of inhibition and a lower FW\u2010Ttpeak ratio than the other groups. The sensitivity of the FibWave was similar to the one of the ETP\u2010based APC resistance assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The FibWave is able to differentiate APC resistance levels observed in women on combined oral contraceptive. The FW\u2010Max<jats:sub>1<\/jats:sub>, FW\u2010Max<jats:sub>2,<\/jats:sub> and to a lesser degree FW\u2010Min<jats:sub>2<\/jats:sub> were identified as the most sensitive parameters with a similar performance to the ETP\u2010based APC resistance assay.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.13427","type":"journal-article","created":{"date-parts":[[2020,12,13]],"date-time":"2020-12-13T07:25:38Z","timestamp":1607844338000},"page":"802-812","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Assessment of acquired activated protein C resistance with the FibWave and comparison with the ETP\u2010based APC resistance"],"prefix":"10.1111","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4255-1591","authenticated-orcid":false,"given":"Jonathan","family":"Evrard","sequence":"first","affiliation":[{"name":"Department of Pharmacy Namur Thrombosis and Hemostasis Center (NTHC) Namur Research Institute for Life Sciences (NARILIS) University of Namur Namur Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laure","family":"Morimont","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Namur Thrombosis and Hemostasis Center (NTHC) Namur Research Institute for Life Sciences (NARILIS) University of Namur Namur Belgium"},{"name":"Qualiblood s.a Namur Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie","family":"Didembourg","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Namur Thrombosis and Hemostasis Center (NTHC) Namur Research Institute for Life Sciences (NARILIS) University of Namur Namur Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00e9l\u00e8ne","family":"Haguet","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Namur Thrombosis and Hemostasis Center (NTHC) Namur Research Institute for Life Sciences (NARILIS) University of Namur Namur Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5003-6939","authenticated-orcid":false,"given":"Romain","family":"Siriez","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Namur Thrombosis and Hemostasis Center (NTHC) Namur Research Institute for Life Sciences (NARILIS) University of Namur Namur Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Michel","family":"Dogn\u00e9","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Namur Thrombosis and Hemostasis Center (NTHC) Namur Research Institute for Life Sciences (NARILIS) University of Namur Namur Belgium"},{"name":"Namur Biobank\u2010eXchange (NAB\u2010X) University of Namur Namur Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7644-5298","authenticated-orcid":false,"given":"Jonathan","family":"Douxfils","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Namur Thrombosis and Hemostasis Center (NTHC) Namur Research Institute for Life Sciences (NARILIS) University of Namur Namur Belgium"},{"name":"Qualiblood s.a Namur Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,12,12]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)80081-9"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.3.1004"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2003.04443.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1998.01028.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCP90HZNZBHNXBE"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1997.192707.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.14532"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2020.05.008"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001721-199701000-00006"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(96)00146-6"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1999.01302.x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2005.01430.x"},{"key":"e_1_2_8_14_1","unstructured":"European Medicines Agency. 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