{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T08:48:59Z","timestamp":1765961339720,"version":"3.40.5"},"reference-count":26,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,1,7]],"date-time":"2021-01-07T00:00:00Z","timestamp":1609977600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31460294","81860651"],"award-info":[{"award-number":["31460294","81860651"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>To investigate CD44 effects on the adriamycin\u2010resistant in chronic myelogenous leukaemia cells K562, we explored the role of CD44 in the K562 cells migration and apoptosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>GeneChip<jats:sup>\u00ae<\/jats:sup> screening is used for elucidating various chemoresistance\u2010related gene expression in the adriamycin\u2010resistant leukaemia cells K562\/ADR. We constructed K562\/CD44 cells by transfection of an EGFP\u2010SV40\u2010CD44 plasmid, and adriamycin\u2010resistant ability was confirmed by detecting migration and apoptosis\u2010related proteins and mRNA expression using Western blotting and Real\u2010time PCR respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>K562\/CD44 cells were generated by the transfection of an EGFP\u2010SV40\u2010CD44 plasmid with high CD44 expression. mRNA expression levels of CD44 and P\u2010glycoprotein (P\u2010gp), along with the proliferation rate, were increased, while the apoptosis rate of K562\/CD44 cells was decreased. Migration\u2010associated proteins such as MMP\u20102 and MMP\u20109 were upregulated, whereas apoptosis\u2010related protein Bax was downregulated and Bcl\u20102 protein was not significantly altered in the K562\/CD44 cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>CD44 might be involved in adriamycin resistance via regulation of P\u2010gp, MMP\u20102, MMP\u20109, and Bcl\u20102\/Bax.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.13455","type":"journal-article","created":{"date-parts":[[2021,1,10]],"date-time":"2021-01-10T20:43:37Z","timestamp":1610311417000},"page":"983-989","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["CD44 enhances adriamycin resistance in chronic myelogenous leukaemia cells 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