{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T21:56:26Z","timestamp":1786658186141,"version":"build-2736575974"},"reference-count":18,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,1,17]],"date-time":"2021-01-17T00:00:00Z","timestamp":1610841600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>The categorization of mature B cell neoplasms (MBN) infiltrating blood and bone marrow are met with difficulties. The inclusion of CD148 and CD180 in the routine flow cytometry\/FCM panels has been suggested to refine the diagnosis. We studied the discriminatory ability of CD148 and CD180 median fluorescence intensity(MFI), CD148\/CD180 ratio and their expression relative to T cells (CD148<jats:sup>ab\/T<\/jats:sup>, CD180<jats:sup>ab\/T<\/jats:sup>), neutrophils (CD148<jats:sup>ab\/gr<\/jats:sup>, CD180<jats:sup>ab\/gr<\/jats:sup>) and normal B cells (CD148<jats:sup>ab\/n<\/jats:sup>, CD180<jats:sup>ab\/n<\/jats:sup>) in the differentiation of mature B cell neoplasms (MBN) especially non\u2010chronic lymphocytic leukaemia (CLL).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>The flow cytometric (FCM) expression of CD148 and CD180 was studied prospectively in 102 patients (non\u2010CLL; n\u00a0=\u00a072); diagnosed by a comprehensive panel of immunophenotypic and cytogenetic studies. The MFI and ratios were statistically compared across MBNs by Mann\u2010Whitney <jats:italic>U<\/jats:italic> test. Cut\u2010off values, sensitivity and specificity were calculated for significant parameters by receiver operator characteristic curve.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CD180MFI\u00a0&gt;\u00a04.35 showed 100% sensitivity and 90.9% specificity for a diagnosis of marginal zone lymphoma (MZL) while, CD148\/180\u00a0&gt;\u00a05.15 was 100% specific and 81.8% sensitive for lymphoplasmacytic lymphoma. CD148<jats:sup>ab\/T<\/jats:sup>(&gt;4.3; 100% specificity, 83.4% sensitivity) and CD148<jats:sup>ab\/gr<\/jats:sup>(&gt;1.1; 100% sensitivity, 90% sensitivity) were useful for differentiating blastoid\u2010mantle cell lymphoma\/MCL from diffuse large B cell lymphoma; while CD148MFI (\u226520.25), CD148<jats:sup>ab\/T<\/jats:sup>(&gt;3.35) and CD148<jats:sup>ab\/gr<\/jats:sup>(&gt;0.95) showed &gt;90% specificity and sensitivity for distinguishing MCL from CLL. Pairwise analysis also showed a good discriminant function of various parameters for distinguishing SMZL from other MBNs like FL, MCL as well as CLL.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The current study shows an excellent utility of CD148MFI, CD180MFI, their ratio and relative expression levels in the subcategorization of immunophenotypically related MBNs.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.13467","type":"journal-article","created":{"date-parts":[[2021,1,17]],"date-time":"2021-01-17T12:01:35Z","timestamp":1610884895000},"page":"1123-1131","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["The relative expression levels of CD148 and CD180 on clonal B cells and CD148\/CD180 median fluorescence intensity ratios are useful in the characterization of mature B cell lymphoid neoplasms infiltrating blood and bone marrow \u2013 Results from a single centre pilot study"],"prefix":"10.1111","volume":"43","author":[{"given":"Arambam","family":"Gautam","sequence":"first","affiliation":[{"name":"Department of Hematology Post Graduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2626-4154","authenticated-orcid":false,"given":"Sreejesh","family":"Sreedharanunni","sequence":"additional","affiliation":[{"name":"Department of Hematology Post Graduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Man Updesh Singh","family":"Sachdeva","sequence":"additional","affiliation":[{"name":"Department of Hematology Post Graduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia","family":"Rana","sequence":"additional","affiliation":[{"name":"Department of Hematology Post Graduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dharambir","family":"Kashyap","sequence":"additional","affiliation":[{"name":"Department of Histopathology Post Graduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parveen","family":"Bose","sequence":"additional","affiliation":[{"name":"Department of Hematology Post Graduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanjeet","family":"Bal","sequence":"additional","affiliation":[{"name":"Department of Histopathology Post Graduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaurav","family":"Prakash","sequence":"additional","affiliation":[{"name":"Adult Clinical Hematology and Bone Marrow Transplantation Unit, Department of Internal Medicine Post Graduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pankaj","family":"Malhotra","sequence":"additional","affiliation":[{"name":"Adult Clinical Hematology and Bone Marrow Transplantation Unit, Department of Internal Medicine Post Graduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8938-5423","authenticated-orcid":false,"given":"Reena","family":"Das","sequence":"additional","affiliation":[{"name":"Department of Hematology Post Graduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1170-5976","authenticated-orcid":false,"given":"Neelam","family":"Varma","sequence":"additional","affiliation":[{"name":"Department of Hematology Post Graduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,1,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V91.8.2800.2800_2800_2809"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V126.23.2662.2662"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21325"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1179\/1607845415Y.0000000005"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-0039.1999.540506.x"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2013.3"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3109\/10428194.2015.1030642"},{"key":"e_1_2_11_9_1","first-page":"7494","article-title":"Detection of t(12;14)(p13;q32) in a patient with IGH\u2010CCND1 negative mantle cell lymphoma resembling ultra\u2010high risk chronic lymphocytic leukemia. 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