{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T02:18:34Z","timestamp":1791598714186,"version":"4.3.4"},"reference-count":16,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,6,29]],"date-time":"2021-06-29T00:00:00Z","timestamp":1624924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Chronic myeloid leukemia (CML) usually presents with leukocytosis with neutrophilia, left shift, and basophilia. Documentation of the <jats:italic>BCR\u2010ABL1<\/jats:italic> fusion is required for diagnosis, and this is often achieved via p210 <jats:italic>BCR\u2010ABL1<\/jats:italic> real\u2010time polymerase chain reaction (RT\u2010PCR).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Patients undergoing first\u2010time testing for p210 <jats:italic>BCR\u2010ABL1<\/jats:italic> at our institution were retrospectively identified. The medical record was reviewed, and the patient age, sex, clinical indication for testing, and concurrent CBC with differential were identified for 518 patients. <jats:italic>BCR\u2010ABL1<\/jats:italic> p210 testing had been performed using a laboratory\u2010developed quantitative RT\u2010PCR assay. Statistical analysis of the results was performed using an unpaired t test, and <jats:italic>P<\/jats:italic> values of &lt;.05 were considered statistically significant.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Twenty\u2010four patients received a new diagnosis of CML (4.6%). As compared to patients with a negative PCR, these patients were more likely to have a markedly elevated white blood cell count (WBC), neutrophilia, and a mild anemia. Ninety\u2010two percent (22\/24) of new CML patients had a WBC \u226520\u00a0\u00d7\u00a010<jats:sup>9<\/jats:sup>\/L, and the two new CML patients with WBC &lt;20\u00a0\u00d7\u00a010<jats:sup>9<\/jats:sup>\/L had basophilia in the peripheral blood. By contrast, 92% (449\/490) of non\u2010CML patients had a WBC &lt;20\u00a0\u00d7\u00a010<jats:sup>9<\/jats:sup>\/L.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The peripheral blood parameters of total WBC \u226520\u00a0\u00d7\u00a010<jats:sup>9<\/jats:sup>\/L and absolute basophil count can help guide the need for <jats:italic>BCR\u2010ABL1<\/jats:italic> PCR testing, which can lead to more judicious test utilization, decreased healthcare costs, and decreased false positives, while keeping a high sensitivity for CML. This study also underscores the importance of obtaining a complete differential in patients for whom CML is suspected.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.13635","type":"journal-article","created":{"date-parts":[[2021,6,29]],"date-time":"2021-06-29T15:12:48Z","timestamp":1624979568000},"page":"1458-1464","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["<i>BCR\u2010ABL1<\/i> p210 screening for chronic myeloid leukemia in patients with peripheral blood cytoses"],"prefix":"10.1111","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9523-9222","authenticated-orcid":false,"given":"Elena","family":"Fenu","sequence":"first","affiliation":[{"name":"Department of Pathology Wake Forest Baptist Medical Center Winston\u2010Salem NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stacey S.","family":"O\u2019Neill","sequence":"additional","affiliation":[{"name":"Department of Pathology Wake Forest Baptist Medical Center Winston\u2010Salem NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Insuasti\u2010Beltran","sequence":"additional","affiliation":[{"name":"Department of Pathology Wake Forest Baptist Medical Center Winston\u2010Salem NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,6,29]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/300765a0"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12032-012-0348-z"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.6004\/jnccn.2020.0047"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-07-1112"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403136"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V76.3.489.489"},{"key":"e_1_2_9_8_1","volume-title":"WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues (International Agency for Research on Cancer, Lyon)","author":"Swerdlow SH","year":"2017"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12185-015-1777-1"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1200\/JOP.2016.014449"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2018.08.038"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V86.8.3118.3118"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V92.9.3362"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1159\/000501146"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa062867"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.14694\/EdBook_AM.2015.35.e381"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.beha.2016.10.005"}],"container-title":["International Journal of Laboratory Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.13635","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ijlh.13635","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.13635","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T08:53:19Z","timestamp":1693212799000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ijlh.13635"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,29]]},"references-count":16,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["10.1111\/ijlh.13635"],"URL":"https:\/\/doi.org\/10.1111\/ijlh.13635","archive":["Portico"],"relation":{},"ISSN":["1751-5521","1751-553X"],"issn-type":[{"value":"1751-5521","type":"print"},{"value":"1751-553X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,29]]},"assertion":[{"value":"2020-08-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}