{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:15:38Z","timestamp":1747185338203,"version":"3.40.5"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,9,7]],"date-time":"2021-09-07T00:00:00Z","timestamp":1630972800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004329","name":"Javna Agencija za Raziskovalno Dejavnost RS","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Philadelphia chromosome (Ph)\u2010like acute lymphoblastic leukemia (ALL) is a biologically and clinically challenging subtype of B\u2010cell ALL which has been incorporated into the 2016 revision of the World Health classification of acute leukemia. It is independently associated with poor outcome. As it can only be reliably detected by expression profiling, it is difficult to diagnose with routine methods. Its recognition has become of greater importance due to prognostication and even more due to the new diagnostic options given by targeted therapies. There is still no standardized diagnostic test enabling its prompt recognition. Here, we introduce our approach how to detect it by combination of widely available techniques.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>179 ALL patients diagnosed in our center during the last 8\u00a0years were included. Data on immunophenotype and cytogenetics were used to select patients with potentially Ph\u2010like ALL (65\/179). <jats:italic>CRLF2<\/jats:italic> gene rearrangement (<jats:italic>CRLF2\u2010r<\/jats:italic>) was tested by FISH in 59\/65 patients, and next\u2010generation sequencing was done by Archer FusionPlex ALL kit in 34 patients. TSLPR expression was determined in 20 patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Philadelphia chromosome\u2010like aberrations were confirmed in 9 patients. In 10% of tested samples, <jats:italic>CRLF2\u2010r<\/jats:italic> was confirmed. Due to a lack of material, NGS was done only in a half of potentially Ph\u2010like cases. In 10%, other Ph\u2010like fusions were found by NGS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The obtained frequencies, and genetic and patients' characteristics are in concordance with the literature data, ensuring a reliable detection of this challenging ALL subtype. The proposed algorithm allows detection of Ph\u2010like ALL at reasonable cost and acceptable workload.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.13698","type":"journal-article","created":{"date-parts":[[2021,9,7]],"date-time":"2021-09-07T15:17:44Z","timestamp":1631027864000},"page":"142-149","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Recognition of Philadelphia chromosome\u2010like acute lymphoblastic leukemia as part of routine diagnostic work\u2010up"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3752-2067","authenticated-orcid":false,"given":"Helena","family":"Podgornik","sequence":"first","affiliation":[{"name":"Department of Hematology University Medical Centre Ljubljana Ljubljana Slovenia"},{"name":"Faculty of Pharmacy University of Ljubljana Ljubljana Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Doplihar Kebe","sequence":"additional","affiliation":[{"name":"Department of Hematology University Medical Centre Ljubljana Ljubljana Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jurka","family":"Klun","sequence":"additional","affiliation":[{"name":"Department of Hematology University Medical Centre Ljubljana Ljubljana Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katarina","family":"Reber\u0161ek","sequence":"additional","affiliation":[{"name":"Department of Hematology University Medical Centre Ljubljana Ljubljana Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"\u0160u\u0107urovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Hematology University Medical Centre Ljubljana Ljubljana Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matev\u017e","family":"\u0160kerget","sequence":"additional","affiliation":[{"name":"Department of Hematology University Medical Centre Ljubljana Ljubljana Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samo","family":"Zver","sequence":"additional","affiliation":[{"name":"Department of Hematology University Medical Centre Ljubljana Ljubljana Slovenia"},{"name":"Medical Faculty University of Ljubljana Ljubljana Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,9,7]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(08)70339-5"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0808253"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-03-643544"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1403088"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-07-726588"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2017-06-743252"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2016.69.0073"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2014.117424"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2015.136366"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2012.06.005"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2017.06.012"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-12-758979"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2015.136366"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2011.40.3907"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-11-256131"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.beha.2017.07.003"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.31848"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.beha.2019.101096"},{"key":"e_1_2_9_20_1","unstructured":"http:\/\/quiver.archerdx.com\/results?query. 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