{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T00:01:05Z","timestamp":1750204865533,"version":"3.40.5"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,1,5]],"date-time":"2022-01-05T00:00:00Z","timestamp":1641340800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Type 3 von Willebrand disease (VWD) is a rare autosomal recessive disorder characterized by undetectable von Willebrand Antigen (VWF:Ag). Carriers of type 3 VWD carry one null allele and have von Willebrand factor (VWF) at about 50% of normal. The aim of this study was to characterize type 3 VWD carriers and to study the role of Platelet Function Analyzer (PFA\u2010200) in this cohort.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This was a cross\u2010sectional study where data were collected from carriers (parents\/offspring) of type 3 VWD patients and evaluated with activated partial thromboplastin time, factor VIII, blood group, ristocetin cofactor assay (VWF:RCo), VWF:Ag, and closure time on PFA\u2010200 with collagen\/epinephrine (COL\/EPI), and collagen\/ADP (COL\/ADP).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>One hundred carriers were included in the study of which 85 were included for PFA\u2010200 analysis. The mean (SD) of VWF:Ag (IU\/ml) and VWF:RCo (IU\/ml) was 0.63 (0.24) and 0.61 (0.26), respectively. Among the 100 carriers, based on VWF levels (VWF:Ag and\/or VWF:RCo) and bleeding history, there were 7 type 1 VWD, 10 type 2 VWD, 25 borderline VWF (0.30\u20130.50\u00a0IU\/ml and no bleeding), and 58 normal VWF (&gt;0.50\u00a0IU\/ml). PFA\u2010200 was prolonged in 71% of the carriers, all carriers with type 1 and type 2 VWD phenotype, 80% carriers with borderline VWF, and 59% with normal VWF. COL\/EPI was more sensitive than COL\/ADP and showed better correlation with VWF parameters than COL\/ADP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Carriers of type 3 VWD can have a variable laboratory phenotype. PFA\u2010200 showed good sensitivity among the carriers at VWF levels &lt;0.50\u00a0IU\/ml.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.13787","type":"journal-article","created":{"date-parts":[[2022,1,5]],"date-time":"2022-01-05T23:59:15Z","timestamp":1641427155000},"page":"603-609","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Laboratory characterization of obligate carriers of type 3 von Willebrand disease with a potential role for Platelet Function Analyzer (PFA\u2010200)"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4247-4154","authenticated-orcid":false,"given":"Tulasi","family":"Geevar","sequence":"first","affiliation":[{"name":"Department of Transfusion Medicine and Immunohematology Christian Medical College Vellore Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rutvi 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