{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T16:14:23Z","timestamp":1770912863681,"version":"3.50.1"},"reference-count":11,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,3,21]],"date-time":"2022-03-21T00:00:00Z","timestamp":1647820800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Recently, CD26 have been identified as one of the promising and specific marker for the identification of leukemic stem cells (LSCs) in chronic myeloid leukemia (CML).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This was a prospective, observational validation study. Peripheral blood (PB) samples from suspected cases of CML and other hematolymphoid neoplasm were evaluated for the expression of CD26 on stem cells (SC) (CD45 dim\/CD34+\/CD38\u2010) fraction by flow cytometry (FCM) using a single tube four\u2010color antibodies cocktail: CD45\u2010V500 \/CD26\u2010PE\/CD34\u2010PerCPcy5.5\/CD38\u2010APC\u2010H7. The diagnosis of CML was confirmed using cytogenetics and\/or molecular studies. Additionally, 12 paired PB and bone marrow (BM) samples of CML cases were compared for the proportion of CD26+ LSCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Expression of CD26 on the SC fraction was invariably noted in all cases (116\/116) of CML, irrespective of the disease phase and transcript type. None of other neoplasm (0\/26), including the Ph\u00a0+\u00a0ALLs expressed CD26. Proportion of SCs expressing CD26 was variable with a median (range) proportion being 61.3% (7.6%\u201398.6%). Evaluation of paired PB and BM samples showed similar proportion of CD26\u00a0+\u00a0LSCs (R<jats:sup>2<\/jats:sup>: 0.969).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We confirmed that FCM evaluation of CD26 expression in the PB LSCs is a rapid and specific tool for CML diagnosis. Its utility as a marker for residual disease evaluation can also be explored in the future.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.13826","type":"journal-article","created":{"date-parts":[[2022,3,22]],"date-time":"2022-03-22T05:38:13Z","timestamp":1647927493000},"page":"524-530","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["CD26 expression on circulating CD34+\/CD38\u2010 progenitor population is a specific and reliable tool for the rapid flow cytometric diagnosis of chronic myeloid leukemia\u2014A single\u2010center validation study"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8285-2092","authenticated-orcid":false,"given":"Khaliqur","family":"Rahman","sequence":"first","affiliation":[{"name":"Department of Hematology Sanjay Gandhi Postgraduate Institute of Medical Sciences  Lucknow 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