{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:16:03Z","timestamp":1747185363383,"version":"3.40.5"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T00:00:00Z","timestamp":1649808000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Chimeric antigen receptor (CAR) T cell products are available to treat relapsed\/refractory B\u2010lymphoblastic leukaemia\/lymphoma (B\u2010ALL), diffuse large B\u2010cell lymphoma, mantle\u2010cell lymphoma, and myeloma. CAR products vary by their target epitope and constituent molecules. Hence, there are no common laboratory assays to assess CAR T cell expansion in the clinical setting. We investigated the utility of common haematology laboratory parameters to measure CAR T cell expansion and response.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Archived CellaVision images, absolute lymphocyte counts, and Sysmex CPD parameters spanning 1\u00a0month after CD19\u2010CAR, UCAR19, CD22\u2010CAR, CD33\u2010CAR, and UCAR123 therapy were compared against donor lymphocyte infused control patients. Additionally, CellaVision images gathered during acute EBV infection were analysed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CellaVision images revealed a distinct sequence of three lymphocyte morphologies, common among CD19\u2010CAR, CD22\u2010CAR and UCAR19. This lymphocyte sequence was notably absent in CAR T cell non\u2010responders and stem\u2010cell transplantation controls, but shared some features seen during acute EBV infection. CD19\u2010CAR engraftment kinetics monitored by quantitative PCR show an expansion and persistence phase and mirror CD19\u2010CAR ALC kinetics. We show other novel CAR T cell therapies (UCAR19, CD22\u2010CAR, CD33\u2010CAR and UCAR123) display similar ALC expansion in responders and diminished ALC expansion in non\u2010responders. Furthermore, the CPD parameter LY_WY fluorescence increased within the first week after CD19\u2010CAR infusion, preceding the peak absolute lymphocyte count (ALC) by 3.7\u2009days.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Autologous and allogeneic CAR T cell therapy produce unique changes in common haematology laboratory parameters and could be a useful surrogate to follow CAR T\u2010cell expansion after infusion.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.13850","type":"journal-article","created":{"date-parts":[[2022,4,14]],"date-time":"2022-04-14T05:59:21Z","timestamp":1649915961000},"page":"750-758","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Haematology laboratory parameters to assess efficacy of <scp>CD19<\/scp>\u2010, <scp>CD22<\/scp>\u2010, <scp>CD33<\/scp>\u2010, and <scp>CD123<\/scp>\u2010directed <scp>c<\/scp>himeric antigen receptor <scp>T\u2010cell<\/scp> therapy in haematological malignancies"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1134-4779","authenticated-orcid":false,"given":"Bradley","family":"Drumheller","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Pennsylvania  Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kirubel","family":"Gebre","sequence":"additional","affiliation":[{"name":"University of Pennsylvania Perelman School of Medicine  Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian","family":"Lockhart","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Children's Hospital of Philadelphia  Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth","family":"Margolskee","sequence":"additional","affiliation":[{"name":"Department of Pathology and 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