{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T15:11:25Z","timestamp":1787497885393,"version":"build-2736575974"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,4,20]],"date-time":"2022-04-20T00:00:00Z","timestamp":1650412800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81774080"],"award-info":[{"award-number":["81774080"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Acute graft\u2010versus\u2010host disease (aGVHD) is one of the major complications of allogeneic hematopoietic stem cell transplantation, and the liver, skin, and gastrointestinal tract are the main target organs. The most common type is intestinal aGVHD. Long noncoding RNAs (lncRNAs) have coregulatory functions and participate in a variety of intracellular regulatory processes. We investigated the expression of lncRNAs and their mechanisms in the development of aGVHD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The participants included 15 patients with aGVHD and 4 healthy controls (HCs). To generate profiles of abnormally expressed lncRNAs, peripheral blood mononuclear cell (PBMC) lncRNAs from four patients and four HCs were validated by high\u2010throughput sequencing and quantitative real\u2010time\u2010PCR (qRT\u2010PCR). A number of databases, including Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, miRanda, TargetScan, and Metascape, were used for bioinformatics analysis. Bioinformatics analysis indicated that overexpression of lnc\u2010AC145676.2.1\u20106\u20103 might induce aGVHD via the interleukin (IL)\u20101\u03b2 axis and a downstream miRNA. After the higher levels of lnc\u2010AC145676.2.1\u20106\u20103 in other patients were confirmed by qRT\u2010PCR, serum IL\u20101\u03b2, IL\u20106, and tumor necrosis factor\u2010\u03b1 were measured by enzyme linked immunosorbent assays.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In our study, a large number of lncRNAs were found in PBMCs of patients with intestinal aGVHD, and bioinformatics analysis showed that the upregulated lncRNA lnc\u2010AC145676.2.1\u20106\u20103 probably affected the progression of intestinal aGVHD by regulating the hsa\u2010miR\u20103064\u20105p\/IL\u20101\u03b2 axis. In addition, the changes in lncRNA expression levels were positively correlated with the clinical characteristics of intestinal aGVHD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our results suggest that lncRNAs in PBMCs may become new biomarkers and therapeutic targets for intestinal aGVHD.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ijlh.13852","type":"journal-article","created":{"date-parts":[[2022,4,20]],"date-time":"2022-04-20T03:05:53Z","timestamp":1650423953000},"page":"759-768","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp>lnc\u2010AC145676<\/scp>\n                    .2.1\u20106\u20103 plays an important role in intestinal acute graft\u2010versus\u2010host disease through the regulation of interleukin\u20101\u03b2"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4692-8309","authenticated-orcid":false,"given":"Xiaoqi","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Traditional Chinese Medicine Shandong University of Traditional, Chinese Medicine  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengting","family":"Xia","sequence":"additional","affiliation":[{"name":"The First Clinical Medical College Shandong University of Traditional Chinese Medicine  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Osteoporosis Affiliated Hospital of Shandong University of Traditional Chinese Medicine  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinwei","family":"Cui","sequence":"additional","affiliation":[{"name":"Jingwu Road Primary School of Jinan  Jinan 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