{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T10:48:03Z","timestamp":1780656483570,"version":"3.54.1"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,8,11]],"date-time":"2022-08-11T00:00:00Z","timestamp":1660176000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Mild anaemia often occurs in the third trimester of pregnancy. Particularly in the Hb range between 101\u2013110\u2009g\/L it is difficult to determine whether the decreased haemoglobin concentration is physiological or pathological and whether supplementation is required.<\/jats:p><jats:p>The aim of this study was to gain insight into the added value of measuring the percentages of microcytic RBCs (%MicroR) and hypochromic RBCs (%Hypo\u2010He) for monitoring effects of iron supplementation in case of suspected iron\u2010deficient erythropoiesis (IDE) in the third trimester of pregnancy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>After assessing haematological parameters and zinc protoporphyrin\/heme ratio as marker for IDE, subjects were classified into a group with symptoms of IDE (<jats:italic>n<\/jats:italic>\u00a0=\u00a039) or without IDE (<jats:italic>n<\/jats:italic>\u00a0=\u00a0106). The subjects with IDE (<jats:italic>n<\/jats:italic>\u00a0=\u00a039) were treated with iron supplementation. After 4\u2009weeks effects of treatment were evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the group of pregnant women with IDE results of %MicroR and %Hypo\u2010He were increased (<jats:italic>p<\/jats:italic>\u00a0=\u00a0&lt;.001), compared to the group without haematological symptoms of IDE, whereas RET\u2010He, RBC\u2010He and delta\u2010He were decreased (<jats:italic>p<\/jats:italic>\u00a0=\u00a0&lt;.001).<\/jats:p><jats:p>A significant positive correlation to increased values of %MicroR (<jats:italic>r<\/jats:italic>\u00a0=\u00a0.75,<jats:italic>p<\/jats:italic>\u00a0=\u00a0&lt;.001) and %Hypo\u2010He (<jats:italic>r<\/jats:italic>\u00a0=\u00a0.77,<jats:italic>p<\/jats:italic>\u00a0=\u00a0&lt;.001) with ZPP was established. However, in the ZPP interval 75\u2013100\u2009\u03bcmol\/mol heme a slight overlap was demonstrated between subjects with and without symptoms of IDE.<\/jats:p><jats:p>After iron supplementation, %Hypo\u2010He decreased (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.002) while %MicroR remained stable. RET\u2010He, delta\u2010He and RDW\u2010<jats:italic>SD<\/jats:italic>increased (<jats:italic>p<\/jats:italic>\u00a0=\u00a0&lt;.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The added value of %MicroR and %Hypo\u2010He as a single marker for IDE is poor. However, combined interpretation of %MicroR, %Hypo\u2010He, Ret\u2010He and delta\u2010He has added value in monitoring erythropoiesis during pregnancy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.13948","type":"journal-article","created":{"date-parts":[[2022,8,12]],"date-time":"2022-08-12T03:04:29Z","timestamp":1660273469000},"page":"1060-1067","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Effects of iron supplementation on microcytic and hypochromic red blood cells during the third trimester of pregnancy"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2114-6888","authenticated-orcid":false,"given":"Margreet","family":"Schoorl","sequence":"first","affiliation":[{"name":"Northwest Clinics, Department of Clinical Chemistry Hematology &amp; Immunology Alkmaar The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1490-7052","authenticated-orcid":false,"given":"Marianne","family":"Schoorl","sequence":"additional","affiliation":[{"name":"Northwest Clinics, Department of Clinical Chemistry Hematology &amp; Immunology Alkmaar The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,8,11]]},"reference":[{"key":"e_1_2_12_2_1","first-page":"91","article-title":"Pregnancy and iron deficiency: unresolved issues","volume":"55","author":"Lindsay H","year":"1997","journal-title":"Nutr Rev"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1079\/PHN2001139"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-007-0305-7"},{"key":"e_1_2_12_5_1","unstructured":"De BenoistB McLeanE EgliI CogswellM.World prevalence of anaemia 1993\u20132005; WHO Global Database on anaemia. 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