{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T11:46:18Z","timestamp":1783943178354,"version":"3.55.0"},"reference-count":10,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,9,3]],"date-time":"2022-09-03T00:00:00Z","timestamp":1662163200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Established parameters for the investigation of suspected iron deficiency have recognized limitations that affect their sensitivity and specificity. Reticulocyte haemoglobin content (RHC) is an early biomarker of iron deficiency or restriction, which also demonstrates response to iron therapy. RHC parameters are offered on all major automated haematology analysers. There is no external quality assessment (EQA) for RHC in the UK, restricting its wider clinical application. The UK National External Quality Assessment Scheme (UKNEQAS) for Haematology has completed a pilot study on the use of stabilized blood for RHC EQA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Blood was obtained from two <jats:italic>JAK2 V617F mutation<\/jats:italic>\u2010positive, polycythaemia vera patients undergoing regular venesection, and from a healthy volunteer donor. Aliquots of each donation were distributed to users of Abbott, Horiba, Siemens and Sysmex automated analysers for RHC analysis on days 1, 3, 5 and 7.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Results were received from 20 laboratories using four different platforms. The daily mean and standard deviation (SD) were calculated for each aliquot, by day of analysis and platform. With RHC there was no significant within\u2010platform difference (<jats:italic>p<\/jats:italic>\u00a0&gt;\u20090.5) between testing days, although there were statistically significant differences (<jats:italic>p<\/jats:italic>\u00a0&lt;\u20090.001) between different platforms. The greatest difference was seen between Abbott MCHr and Horiba RhCc (\u22126.14\u2009\u00b1\u20091.25\u2009pg). Despite inter\u2010platform differences, it was possible to define iron deficient, borderline, and normal cut\u2010offs to classify 95% of results correctly.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The results demonstrate that it is possible to provide RHC EQA material using UKNEQAS standard procedures. The results are clinically relevant but indicate a requirement for inter\u2010user comparison.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.13957","type":"journal-article","created":{"date-parts":[[2022,9,3]],"date-time":"2022-09-03T08:23:26Z","timestamp":1662193406000},"page":"1088-1093","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp>UK NEQAS<\/scp> Haematology pilot scheme: Reticulocyte haemoglobin content quantitation"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4141-4811","authenticated-orcid":false,"given":"Kathryn E.","family":"Mordue","sequence":"first","affiliation":[{"name":"University Hospitals Plymouth NHS Trust  Plymouth UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne","family":"Hector","sequence":"additional","affiliation":[{"name":"UK NEQAS Haematology West Hertfordshire Hospitals NHS Trust  Watford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3349-947X","authenticated-orcid":false,"given":"D. Wayne","family":"Thomas","sequence":"additional","affiliation":[{"name":"University Hospitals Plymouth NHS Trust  Plymouth UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2926-7441","authenticated-orcid":false,"given":"Barbara J.","family":"De La Salle","sequence":"additional","affiliation":[{"name":"UK NEQAS Haematology West Hertfordshire Hospitals NHS Trust  Watford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,9,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.17900"},{"key":"e_1_2_8_3_1","first-page":"1","article-title":"British Society of Gastroenternology guidelines for the management of iron deficiency anaemia in adults","volume":"0","author":"Snook J","year":"2021","journal-title":"Gut"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.12311"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.16221"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.ajkd.2015.11.012"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V85.3.818.bloodjournal853818"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12514"},{"key":"e_1_2_8_9_1","first-page":"19","article-title":"A whole blood control for blood count analysers and a source material for an EQA scheme","volume":"48","author":"Reardon DM","year":"1991","journal-title":"Med Lab Sci"},{"key":"e_1_2_8_10_1","first-page":"1133","article-title":"Clinical utility of the new Sysmex XE 2100 parameter\u2010reticulocyte haemoglobin equivalent\u2010in the diagnosis of anaemia","volume":"90","author":"Canals C","year":"2005","journal-title":"Haematologica"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12262"}],"container-title":["International Journal of Laboratory Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.13957","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ijlh.13957","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.13957","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T04:06:34Z","timestamp":1692677194000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ijlh.13957"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,3]]},"references-count":10,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["10.1111\/ijlh.13957"],"URL":"https:\/\/doi.org\/10.1111\/ijlh.13957","archive":["Portico"],"relation":{},"ISSN":["1751-5521","1751-553X"],"issn-type":[{"value":"1751-5521","type":"print"},{"value":"1751-553X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,3]]},"assertion":[{"value":"2022-05-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}