{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:16:17Z","timestamp":1747185377499,"version":"3.40.5"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,9,16]],"date-time":"2022-09-16T00:00:00Z","timestamp":1663286400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>The erythrocyte sedimentation rate (ESR) includes three phases, each prone to different interferences. Due to many disadvantages of the reference Westergren method, modified and alternate methods have been introduced. The aim of this study was to compare the modified Westergren method on SRS 100\/II analyzer in citrate blood with the alternate method on iSED\u00ae analyzer in EDTA sample. Additionally, possible interfering factors and ESR stability during 6\u00a0h at room temperature were evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 188 samples were included in the method comparison. Additionally, the effects of inflammation, haematocrit and MCV values on ESR were evaluated. To determine ESR stability in different samples, ESR was evaluated at three time points; within 15\u2009min of blood sampling and after 3 and 6\u00a0h in different sample types and analyzers (<jats:italic>N<\/jats:italic>\u00a0=\u200965).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Results indicated the constant difference between tested methods with obtained mean bias of 5\u2009mm (95% CI: 3\u20137). There was higher absolute mean bias in groups with ESR\u00a0&gt;\u200940\u2009mm and elevated inflammation markers (<jats:italic>p<\/jats:italic>\u00a0&lt;\u20090.001). Regarding different MCV and haematocrit groups there was no statistically significant difference in obtained absolute mean biases for MCV (<jats:italic>p<\/jats:italic>\u00a0=\u20090.087) while there was higher bias in low haematocrit group compared to normal haematocrit (<jats:italic>p<\/jats:italic>\u00a0=\u20090.004). In addition, there was a significant difference between ESR values at different time points for iSED\u00ae (<jats:italic>p<\/jats:italic>\u00a0&lt;\u20090.001) and no difference for SRS 100\/II analyzer (<jats:italic>p<\/jats:italic>\u00a0=\u20090.406).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>There are differences in ESR values between tested methods. EDTA sample on iSED\u00ae should be analysed as soon as possible to avoid falsely increased ESR.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.13970","type":"journal-article","created":{"date-parts":[[2022,9,17]],"date-time":"2022-09-17T00:54:05Z","timestamp":1663376045000},"page":"29-36","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Performance comparison of Westergren modified <scp>SRS<\/scp> 100\/<scp>II<\/scp> and alternate <scp>iSED<\/scp>\u00ae method for erythrocyte sedimentation rate"],"prefix":"10.1111","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0609-0206","authenticated-orcid":false,"given":"Tina","family":"Bren\u010di\u0107","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry and Laboratory Medicine General Hospital Pula  Pula Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gordana","family":"Juri\u010di\u0107","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Laboratory Medicine General Hospital Pula  Pula Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorena","family":"Honovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Laboratory Medicine General Hospital Pula  Pula Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,9,16]]},"reference":[{"key":"e_1_2_11_2_1","first-page":"214","article-title":"Physiological basis and clinical utility of erythrocyte sedimentation rate","volume":"22","author":"Hameed MA","year":"2006","journal-title":"Pak J Med Sci"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2003.019"},{"key":"e_1_2_11_4_1","first-page":"1","article-title":"Comparison of the StaRRsed Interliner device with Westergren method in erythrocyte sedimentation rate measurement","author":"Orkmez M","year":"2021","journal-title":"Int J Lab Hematol"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1258\/acb.2008.008062"},{"key":"e_1_2_11_6_1","first-page":"1","article-title":"Ves\u2010Matic CUBE 200: is modified Westergren method for erythrocyte sedimentation rate a valid alternative to the gold standard?","author":"Lapi\u0107 I","year":"2019","journal-title":"J Clin Pathol"},{"key":"e_1_2_11_7_1","first-page":"198","article-title":"Recommendation for measurement of erythrocyte sedimentation rate","volume":"46","author":"International Committee for Standardization in Hematology","year":"1993","journal-title":"Am J Clin Pathol"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-553X.2011.01302.x"},{"key":"e_1_2_11_9_1","first-page":"50","article-title":"Erythrocyte sedimentation rate \u2013 an old marker with new applicatons","volume":"5","author":"Bochen K","year":"2011","journal-title":"J Pre\u2010Clin Clin Res"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2019-0204"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.22661"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12693"},{"key":"e_1_2_11_13_1","first-page":"1","article-title":"Analytical validation of the iSED automated analyser for erythrocyte sedimentation rate","author":"Lapi\u0107 I","year":"2019","journal-title":"Int J Lab Hematol"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-553X.2008.01135.x"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.21786"},{"key":"e_1_2_11_16_1","unstructured":"Venous Blood Collection. [gbo website]; 2021. Accessed January 15 2022.https:\/\/shop.gbo.com\/en\/row\/products\/preanalytics\/venous-blood-collection\/"},{"key":"e_1_2_11_17_1","unstructured":"VACUETTE\u00ae Automated ESR Systems. [gbo website]; 2021. Accessed January 15 2022.https:\/\/shop.gbo.com\/en\/row\/products\/preanalytics\/instruments\/esr-instruments\/"},{"key":"e_1_2_11_18_1","unstructured":"iSED. [Alcor Scientific website]; 2020. Accessed January 15 2022.https:\/\/alcorscientific.com\/clinical-laboratory\/ised\/"},{"key":"e_1_2_11_19_1","first-page":"11","volume-title":"Harmonizacija laboratorijskih nalaza u podru\u010dju op\u0107e, specijalne i visokodiferentne medicinske biokemije","author":"\u010cvori\u0161\u0107ec D","year":"2007"},{"key":"e_1_2_11_20_1","first-page":"1443","article-title":"Clinical utility of the erythrocyte sedimentation rate","volume":"60","author":"Brigden ML","year":"1999","journal-title":"Am Fam Physician"},{"key":"e_1_2_11_21_1","first-page":"eA11","article-title":"Assessing erythrocyte sedimentation rate in whole blood anticoagulated with sodium citrate or K2EDTA","volume":"5","author":"Salvagno GL","year":"2019","journal-title":"Clin Chem Lab Med"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13104-020-04963-0"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPMEWW62BGQHJH"},{"key":"e_1_2_11_24_1","first-page":"239","article-title":"Comparative analysis of erythrocyte sedimentation rate measured by automated and manual methods in anaemic patients","volume":"12","author":"Narang V","year":"2020","journal-title":"J Lab Phys"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2006.075"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/00365513.2020.1786887"},{"key":"e_1_2_11_27_1","article-title":"Correlation study: Greiner bio one SRT10\/II, SRS20\/II, SRS100\/II 15 minutes reaging compared to 1 hour manual Westergreen method","author":"Eleuteri L","year":"2015","journal-title":"Vital Diagn"}],"container-title":["International Journal of Laboratory Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.13970","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ijlh.13970","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.13970","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T15:06:18Z","timestamp":1692457578000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ijlh.13970"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,16]]},"references-count":26,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,2]]}},"alternative-id":["10.1111\/ijlh.13970"],"URL":"https:\/\/doi.org\/10.1111\/ijlh.13970","archive":["Portico"],"relation":{},"ISSN":["1751-5521","1751-553X"],"issn-type":[{"type":"print","value":"1751-5521"},{"type":"electronic","value":"1751-553X"}],"subject":[],"published":{"date-parts":[[2022,9,16]]},"assertion":[{"value":"2022-05-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}