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We used next\u2010generation sequencing with a 172 gene panel to explore the multi\u2010gene mutational data and the mutational status of immunoglobulin heavy variable (<jats:italic>IGHV<\/jats:italic>) gene.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>MYD88<\/jats:italic> (20.0%) was the most frequently mutated gene, much higher than in Europe, followed in order by <jats:italic>TP53<\/jats:italic> (18.8%), <jats:italic>NOTCH1<\/jats:italic> (14.1%), <jats:italic>IGLL5<\/jats:italic> (11.8%), and <jats:italic>DNMT3A<\/jats:italic> (8.2%). In addition, the incidence of <jats:italic>ATM<\/jats:italic> and <jats:italic>SF3B1<\/jats:italic> mutations was relatively lower in our centre compared to Europe. Mutated (M)\u2010<jats:italic>IGHV<\/jats:italic> patients were more likely to have a cooccurrence of <jats:italic>MYD88<\/jats:italic> mutation, while complex karyotype and <jats:italic>DNMT3A<\/jats:italic> mutation were more common in the unmutated (U)\u2010<jats:italic>IGHV<\/jats:italic> group. <jats:italic>MYD88<\/jats:italic> mutated CLL was characterized by prevalence in young males in high\u2010risk staging, with isolated 13q deletion and concomitant mutation of <jats:italic>IGLL5<\/jats:italic>. CLL patients with <jats:italic>MYD88<\/jats:italic> and <jats:italic>TP53<\/jats:italic> mutation showed an unfavorable prognosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results would be valuable in helping to understand the characteristics and significance of cytogenetic genetics in Chinese patients with CLL.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.14143","type":"journal-article","created":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T08:05:11Z","timestamp":1691481911000},"page":"908-916","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Molecular characteristics in Chinese with chronic lymphocytic leukemia by next\u2010generation sequencing: A single\u2010center retrospective 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