{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T14:47:15Z","timestamp":1760453235682,"version":"3.40.5"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T00:00:00Z","timestamp":1693699200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004351","name":"Sultan Qaboos University","doi-asserted-by":"publisher","award":["IG\/MED\/HAEM\/11\/01"],"award-info":[{"award-number":["IG\/MED\/HAEM\/11\/01"]}],"id":[{"id":"10.13039\/501100004351","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Circulating microparticles (MP) are being described as potential biomarkers for disease activity in a variety of conditions including sickle cell anemia (SCA). However, relatively little is known about the influence of spleen status on MP levels in patients with SCA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Using a prospective study design we characterize circulating MP in 144 patients with SCA in steady state by assessing their cellular origin and their relationships to spleen status defined by clinical and imaging findings. In addition, MP levels were studied according to demographic characteristics, clinical status, treatment modalities, and other hematological and biochemical parameters. Absolute plasma concentrations of MP were determined by flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Patients with SCA displayed a 10\u2010fold increase in levels of MP derived from red blood cell (RBC) and platelets (PLT) when compared to their healthy counterparts (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001). Splenectomized patients with SCA have more pronounced levels of MPRBC and MPPLT, and remained elevated after several weeks of follow\u2010up. Levels of MP were not significantly associated with spleen removal procedures, age, gender, clinical severity score, hydroxyurea therapy, hemoglobin F, and co\u2010existence of glucose\u20106\u2010phosphate dehydrogenase deficiency.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Collectively, these results suggest that splenectomy affects circulating levels of MP regardless of the known SCA modifiers and correlates.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.14162","type":"journal-article","created":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T04:21:25Z","timestamp":1693801285000},"page":"141-147","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Impact of splenectomy on circulating microparticles in patients with sickle cell anemia"],"prefix":"10.1111","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5717-7884","authenticated-orcid":false,"given":"Mohamed\u2010Rachid","family":"Boulassel","sequence":"first","affiliation":[{"name":"Department of Allied Health Sciences College of Medicine and Health Sciences, Sultan Qaboos University  Muscat Sultanate of Oman"},{"name":"Department of Haematology College of Medicine and Health Sciences, Sultan Qaboos University Hospital  Muscat Sultanate of Oman"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khoula","family":"Al\u2010Rubkhi","sequence":"additional","affiliation":[{"name":"Department of Allied Health Sciences College of Medicine and Health Sciences, Sultan Qaboos University  Muscat Sultanate of Oman"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jamal","family":"Al\u2010Qasabi","sequence":"additional","affiliation":[{"name":"Department of Haematology College of Medicine and Health Sciences, Sultan Qaboos University Hospital  Muscat Sultanate of Oman"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Islam","family":"El\u2010Ghamry","sequence":"additional","affiliation":[{"name":"Department of Child Health College of Medicine and Health Sciences, Sultan Qaboos University Hospital  Muscat Sultanate of Oman"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hammad","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Haematology College of Medicine and Health Sciences, Sultan Qaboos University Hospital  Muscat Sultanate of Oman"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vinodh","family":"Panjwani","sequence":"additional","affiliation":[{"name":"Department of Haematology College of Medicine and Health Sciences, Sultan Qaboos University Hospital  Muscat Sultanate of Oman"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rizwan Nabi","family":"Qureshi","sequence":"additional","affiliation":[{"name":"Department of Haematology College of Medicine and Health Sciences, Sultan Qaboos University Hospital  Muscat Sultanate of Oman"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sahimah","family":"Al\u2010Mamari","sequence":"additional","affiliation":[{"name":"Department of Haematology College of Medicine and Health Sciences, Sultan Qaboos University Hospital  Muscat Sultanate of Oman"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zahra","family":"Al\u2010Qarni","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology College of Medicine and Health Sciences, Sultan Qaboos University Hospital  Muscat Sultanate of Oman"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed","family":"Al\u2010Kindi","sequence":"additional","affiliation":[{"name":"Department of Pathology College of Medicine and Health Sciences, Sultan Qaboos 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