{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T17:40:13Z","timestamp":1791222013235,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,12,26]],"date-time":"2023-12-26T00:00:00Z","timestamp":1703548800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009776","name":"McMaster University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009776","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Light transmission aggregometry (LTA) is important for diagnosing platelet function disorders (PFD) and von Willebrand disease (VWD) affecting ristocetin\u2010induced platelet aggregation (RIPA). Nonetheless, data is lacking on the utility of LTA for investigating thrombocytopenic patients and platelet rich plasma samples with low platelet counts (L\u2010PRP). Previously, we developed a strategy for diagnostic LTA assessment of L\u2010PRP that included: (1) acceptance of referrals\/samples, regardless of thrombocytopenia severity, (2) tailored agonist selection, based on which are informative for L\u2010PRP with mildly or severely low platelet counts, and (3) interpretation of maximal aggregation (MA) using regression\u2010derived 95% confidence intervals, determined for diluted control L\u2010PRP (C\u2010L\u2010PRP).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>To further evaluate the L\u2010PRP LTA strategy, we evaluated findings for a subsequent patient cohort.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Between 2008 and 2021, the L\u2010PRP strategy was applied to 211 samples (11.7% of all LTA samples) from 192 unique patients, whose platelet counts (median [range]\u2009\u00d7\u200910<jats:sup>9<\/jats:sup>\/L) for blood and L\u2010PRP were: 105 [13\u2013282; 89% with thrombocytopenia] and 164 [17\u2013249], respectively. Patient\u2010L\u2010PRP had more abnormal MA findings than simultaneously tested C\u2010L\u2010PRP (<jats:italic>p<\/jats:italic>\u2010values &lt;0.001). Among patients with accessible electronic medical records (<jats:italic>n<\/jats:italic>\u2009=\u2009181), L\u2010PRP LTA uncovered significant aggregation abnormalities in 45 (24.9%), including 18\/30 (60%) with &lt;80\u2009\u00d7\u200910<jats:sup>9<\/jats:sup>\u2009platelets\/L\u2009L\u2010PRP, and ruled out PFD, and VWD affecting RIPA, in others. The L\u2010PRP LTA strategy helped diagnose VWD affecting RIPA, Bernard Soulier syndrome, familial platelet disorder with myeloid malignancy, suspected <jats:italic>ITGA2B\/ITGB3<\/jats:italic>\u2010related thrombocytopenia, and acquired PFD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Diagnostic LTA with L\u2010PRP, using a strategy that considers thrombocytopenia severity, is feasible and informative.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.14216","type":"journal-article","created":{"date-parts":[[2023,12,27]],"date-time":"2023-12-27T07:14:45Z","timestamp":1703661285000},"page":"362-374","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Evaluation of a diagnostic platelet aggregation test strategy for platelet rich plasma samples with low platelet counts"],"prefix":"10.1111","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6005-4508","authenticated-orcid":false,"given":"Rahaf Mahmoud","family":"Altahan","sequence":"first","affiliation":[{"name":"Department of Pathology and Molecular Medicine McMaster University  Hamilton Ontario Canada"},{"name":"Pathology and Clinical Laboratory Medicine Administration King Fahad Medical City  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9501-0468","authenticated-orcid":false,"given":"Natalie","family":"Mathews","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine McMaster University  Hamilton Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7323-0104","authenticated-orcid":false,"given":"Alex","family":"Bourguignon","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine McMaster University  Hamilton Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8859-4119","authenticated-orcid":false,"given":"Subia","family":"Tasneem","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine McMaster University  Hamilton Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0943-8853","authenticated-orcid":false,"given":"Donald M.","family":"Arnold","sequence":"additional","affiliation":[{"name":"Department of Medicine McMaster University  Hamilton Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2508-1786","authenticated-orcid":false,"given":"Wendy","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Medicine McMaster University  Hamilton Ontario Canada"},{"name":"Hamilton Regional Laboratory Medicine Program  Hamilton Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2843-0817","authenticated-orcid":false,"given":"Catherine P. 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