{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:23:04Z","timestamp":1777929784085,"version":"3.51.4"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T00:00:00Z","timestamp":1706745600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Dilute Russell's viper venom time (dRVVT) and activated partial thromboplastin time (APTT) are the mainstay assays in lupus anticoagulant (LA) detection yet they have limitations, particularly in relation to interferences and specificity. The recently validated Taipan snake venom time (TSVT) screening with ecarin time (ET) confirmatory assays overcome many of those limitations due to the innate specificity engendered from direct prothrombin activation, and insensitivity to the effects of vitamin K antagonists (VKA). The present study aimed to further evidence diagnostic utility of TSVT\/ET by performing them in samples from 116 nonanticoagulated patients with established triple\u2010positive antiphospholipid syndrome (APS).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Samples were identified in three expert centres who performed dRVVT, APTT and solid phase antiphospholipid antibody assays with reagents from a variety of manufacturers. All samples additionally received TSVT\/ET analysis using standardised reagents.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Ninety seven of 116 (83.6%) were dRVVT\u2010 and APTT\u2010positive, 85\/97 (87.6%) of which were TSVT\/ET\u2010positive, 9\/116 (7.8%) were dRVVT\u2010positive only, 6 of which were TSVT\/ET\u2010positive, and 10\/116 (8.6%) were APTT\u2010positive only, 5 of which were TSVT\/ET\u2010positive. 96\/116 TSVT\/ET\u2010positivity returned a high sensitivity for LA of 82.8%. Low coefficients of determination revealed weak relationships between LA potency and anticardiolipin and anti\u2010\u03b2<jats:sub>2<\/jats:sub>\u2010glycoprotein I antibody titres for all three LA assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>TSVT\/ET has high sensitivity for the clinically significant LA found in triple positive APS patients. TSVT\/ET can establish multiple LA assay positivity in nonanticoagulated patients negative for one of dRVVT or APTT, and is the only assay pairing insensitive to VKAs, the recommended anticoagulation for APS.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.14240","type":"journal-article","created":{"date-parts":[[2024,2,2]],"date-time":"2024-02-02T05:57:20Z","timestamp":1706853440000},"page":"538-545","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Taipan snake venom time has high sensitivity for lupus anticoagulants in non\u2010anticoagulated, triple positive antiphospholipid syndrome patients"],"prefix":"10.1111","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2987-281X","authenticated-orcid":false,"given":"Gary W.","family":"Moore","sequence":"first","affiliation":[{"name":"Department of Haematology, Specialist Haemostasis Unit Cambridge University Hospitals NHS Foundation Trust  Cambridge UK"},{"name":"Department of Natural Sciences, Faculty of Science and Technology Middlesex University  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5466-0448","authenticated-orcid":false,"given":"Sean","family":"Platton","sequence":"additional","affiliation":[{"name":"The Royal London Hospital Haemophilia Centre Barts Health NHS Trust  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-4339-4417","authenticated-orcid":false,"given":"Nada","family":"Yartey","sequence":"additional","affiliation":[{"name":"East and South East London NHS Pathology Partnership Barts Health NHS Trust  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0775-4759","authenticated-orcid":false,"given":"Eleanor","family":"Foxton","sequence":"additional","affiliation":[{"name":"Synnovis Haemostasis and Thrombosis Laboratory St. Thomas' Hospital  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7711-6875","authenticated-orcid":false,"given":"Danielle","family":"White","sequence":"additional","affiliation":[{"name":"Department of Haematology, Specialist Haemostasis Unit Cambridge University Hospitals NHS Foundation Trust  Cambridge UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4944-3289","authenticated-orcid":false,"given":"Stephen G.","family":"MacDonald","sequence":"additional","affiliation":[{"name":"Department of Haematology, Specialist Haemostasis Unit Cambridge University Hospitals NHS Foundation Trust  Cambridge UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2017.07.006"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0042-1744363"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2009.03674.x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/0961203317692432"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-03-340232"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/0961203314545410"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.14846"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.15047"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2012.09037.x"},{"key":"e_1_2_9_11_1","volume-title":"Laboratory Testing for the Lupus Anticoagulant; 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