{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T09:51:54Z","timestamp":1770285114583,"version":"3.49.0"},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,2,21]],"date-time":"2024-02-21T00:00:00Z","timestamp":1708473600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100012505","name":"Royal Perth Hospital Medical Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012505","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Detection of del(17p) in myeloma is generally performed by fluorescence in situ hybridization (FISH) on a slide with analysis of up to 200 nuclei. The small cell sample analyzed makes this a low precision test. We report the utility of an automated FISH method, called \u201cimmuno\u2010flowFISH\u201d, to detect plasma cells with adverse prognostic risk del(17p) in bone marrow and blood samples of patients with myeloma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Bone marrow (<jats:italic>n<\/jats:italic>\u2009=\u200931) and blood (<jats:italic>n<\/jats:italic>\u2009=\u200919) samples from 35 patients with myeloma were analyzed using immuno\u2010flowFISH. Plasma cells were identified by CD38\/CD138\u2010immunophenotypic gating and assessed for the 17p locus and centromere of chromosome 17. Cells were acquired on an AMNIS ImageStreamX MkII imaging flow cytometer using INSPIRE software.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Chromosome 17 abnormalities were identified in CD38\/CD138\u2010positive cells in bone marrow (6\/31) and blood (4\/19) samples when the percent plasma cell burden ranged from 0.03% to 100% of cells. Abnormalities could be identified in 14.5%\u2013100% of plasma cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The \u201cimmuno\u2010flowFISH\u201d imaging flow cytometric method could detect del(17p) in plasma cells in both bone marrow and blood samples of myeloma patients. This method was also able to detect gains and losses of chromosome 17, which are also of prognostic significance. The lowest levels of 0.009% (bone marrow) and 0.001% (blood) for chromosome 17 abnormalities was below the detection limit of current FISH method. This method offers potential as a new means of identifying these prognostically important chromosomal defects, even when only rare cells are present and for serial disease monitoring.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.14248","type":"journal-article","created":{"date-parts":[[2024,2,21]],"date-time":"2024-02-21T08:26:25Z","timestamp":1708503985000},"page":"495-502","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Imaging flow cytometric detection of del(<scp>17p<\/scp>) in bone marrow and circulating plasma cells in multiple myeloma"],"prefix":"10.1111","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8347-4302","authenticated-orcid":false,"given":"Thomas I.","family":"Mincherton","sequence":"first","affiliation":[{"name":"School of Biomedical Sciences The University of Western Australia  Crawley Western Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie J.","family":"Lam","sequence":"additional","affiliation":[{"name":"PathWest Laboratory Medicine  Nedlands Western Australia Australia"},{"name":"Haematology Department Fiona Stanley Hospital  Murdoch Western Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah E.","family":"Clarke","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences The University of Western Australia  Crawley Western Australia Australia"},{"name":"PathWest Laboratory Medicine  Nedlands Western Australia Australia"},{"name":"Haematology Department Fiona Stanley Hospital  Murdoch Western Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0442-6987","authenticated-orcid":false,"given":"Henry Y. L.","family":"Hui","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences The University of Western Australia  Crawley Western Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacques A. J.","family":"Malherbe","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences The University of Western Australia  Crawley Western Australia Australia"},{"name":"Haematology Department Fiona Stanley Hospital  Murdoch Western Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hun S.","family":"Chuah","sequence":"additional","affiliation":[{"name":"PathWest Laboratory Medicine  Nedlands Western Australia Australia"},{"name":"Haematology Department Royal Perth Hospital  Perth Western Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1089-3853","authenticated-orcid":false,"given":"M. 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