{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,1,31]],"date-time":"2025-01-31T05:16:25Z","timestamp":1738300585241,"version":"3.35.0"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,2,23]],"date-time":"2024-02-23T00:00:00Z","timestamp":1708646400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Agarose gel\u2010based conventional and real\u2010time allele\u2010specific polymerase chain reaction (AS\u2010PCR) assays are currently used for sensitive detection and quantification of <jats:italic>MYD88<\/jats:italic> L265P mutation. Visual inspection of an agarose gel can often be ambiguous. We propose a new allele\u2010specific quantification PCR (AS\u2010qPCR) assay, PlentiPlex\u2122 <jats:italic>MYD<\/jats:italic>88 Waldenstr\u00f6m lymphoma qPCR assay, that uses Intercalating Nucleic Acid (INA\u00ae) technology for increased affinity and specificity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This study compares PlentiPlex\u2122 <jats:italic>MYD<\/jats:italic>88 Waldenstr\u00f6m lymphoma qPCR assay with conventional AS\u2010PCR. We included a total of 102 peripheral and bone marrow blood samples from 94 patients with a lymphoproliferative disorder. Droplet digital PCR (ddPCR) was used as a third method in case of discrepancy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A positive percent agreement of 100% (95% CI 0.92\u20131.0) and a negative percent agreement of 98% (95% CI 0.90\u20131.0) were found between the conventional AS\u2010PCR and the AS\u2010qPCR methods. Including the ddPCR results to validate the discrepant cases, the sensitivity and specificity of PlentiPlex\u2122 <jats:italic>MYD<\/jats:italic>88 Waldenstr\u00f6m lymphoma qPCR Assay were 1.0 (95% CI 0.97\u20131.0) and 1.0 (95% CI 0.96\u20131.0), respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our data demonstrate that PlentiPlex\u2122 <jats:italic>MYD<\/jats:italic>88 Waldenstr\u00f6m lymphoma qPCR assay is a fast, highly sensitive, and specific method for the detection of <jats:italic>MYD<\/jats:italic>88 L265P compared with conventional AS\u2010PCR.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.14255","type":"journal-article","created":{"date-parts":[[2024,2,23]],"date-time":"2024-02-23T10:49:32Z","timestamp":1708685372000},"page":"665-669","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>PlentiPlex<\/scp>\u2122 <scp><i>MYD88<\/i><\/scp> Waldenstr\u00f6m lymphoma <scp>qPCR<\/scp> assay: A highly sensitive method for detection of <scp>MYD88 L265P<\/scp> mutation"],"prefix":"10.1111","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3012-6539","authenticated-orcid":false,"given":"Marcello","family":"Viscovo","sequence":"first","affiliation":[{"name":"Sezione di Ematologia, Dipartimento di Scienze Radiologiche ed Ematologiche Universit\u00e0 Cattolica del Sacro Cuore Facolt\u00e0 di Medicina e Chirurgia  Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mia de Laurent","family":"Clemmensen","sequence":"additional","affiliation":[{"name":"PentaBase A\/S  Odense Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federica","family":"Fosso","sequence":"additional","affiliation":[{"name":"Dipartimento di Diagnostica per Immagini, Radioterapia Oncologica ed Ematologia Fondazione Policlinico Universitario A Gemelli IRCC  Rome 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