{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T01:27:07Z","timestamp":1779326827540,"version":"3.51.4"},"reference-count":26,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T00:00:00Z","timestamp":1714608000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T00:00:00Z","timestamp":1714608000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100012774","name":"Innovationsfonden","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012774","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012774","name":"Innovationsfonden","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012774","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>A polyvalent blood collection tube could potentially reduce the number and volume of blood samples drawn from patients and reduce the risk of tube mix\u2010ups in a point\u2010of\u2010care setting in the emergency department and the intensive care unit.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Four different concentrations of our experimental heparin anticoagulant with iloprost additive (HEP\u2010ILOP 50\u2009nM, 150\u2009nM, 1000\u2009nM, and 10\u2009\u03bcM, respectively) were tested for significant differences and bias performance specifications against EDTA for 29 hematology analytes, and the highest concentration (HEP\u2010ILOP 10\u2009\u03bcM) against lithium heparin for 14 chemistry and immunochemistry analytes. Samples were drawn from 79 consenting subjects from the Oncology Department (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200938) and the Intensive and Intermediary Care Unit (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200941).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>For hematology analytes, the HEP\u2010ILOP formulation generally provided stable measurement within optimal requirements within 5\u2009h after sampling (mean 104\u2009\u00b1\u200956\u2009min), with very little difference between the four HEP\u2010ILOP concentrations. Because of differences in platelet and red blood cell swelling between EDTA and HEP\u2010ILOP, all size\u2010dependent analytes required proportional factorization to produce similar results. Platelet count by impedance similarly required factorization, whereas the fluorescent method provided results identical with EDTA. Chemistry and immunochemistry analytes were within optimal requirements except for potassium, lactate dehydrogenase, and glucose, indicating a cytoprotective effect of iloprost reducing cell metabolism and rupture, thereby producing results closer to in vivo conditions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our novel dry\u2010sprayed anticoagulant formulation, HEP\u2010ILOP, is a promising candidate for a polyvalent blood collection tube, enabling the analysis of hematology, chemistry, and immunochemistry analytes in the same tube.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ijlh.14291","type":"journal-article","created":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T05:14:24Z","timestamp":1714626864000},"page":"809-821","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Stable measurement of chemistry, immunochemistry, and hematology analytes in a heparin\u2010based anticoagulant with iloprost additive: A promising candidate for the polyvalent blood collection tube"],"prefix":"10.1111","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2695-6337","authenticated-orcid":false,"given":"Elias Frost","family":"Wiwe","sequence":"first","affiliation":[{"name":"Department of Clinical Biochemistry, Nordsj\u00e6llands Hospital Copenhagen University Hospital  Hiller\u00f8d Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura Emilie","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, Nordsj\u00e6llands Hospital Copenhagen University Hospital  Hiller\u00f8d Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emilie","family":"Zeuthen","sequence":"additional","affiliation":[{"name":"Department of Anesthesia and Intensive Care, Nordsj\u00e6llands Hospital Copenhagen University Hospital  Hiller\u00f8d Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ida Maria Storm","family":"Engelstoft","sequence":"additional","affiliation":[{"name":"Department of Oncology and Palliative Care, Nordsj\u00e6llands Hospital Copenhagen University Hospital  Hiller\u00f8d Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Buris Brinch","family":"Christiansen","sequence":"additional","affiliation":[{"name":"Department of Oncology and Palliative Care, Nordsj\u00e6llands Hospital Copenhagen University Hospital  Hiller\u00f8d Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas Steen","family":"Hansen","sequence":"additional","affiliation":[{"name":"Radiometer Medical ApS  Br\u00f8nsh\u00f8j Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melanie A.","family":"Burkhardt","sequence":"additional","affiliation":[{"name":"Radiometer Medical ApS  Br\u00f8nsh\u00f8j Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claus Antonio Juel","family":"Jensen","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, Nordsj\u00e6llands Hospital Copenhagen University Hospital  Hiller\u00f8d Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,5,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408363.2017.1399336"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/POC.0b013e31821c6bb3"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.17219\/acem\/32065"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2010.191"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2022.10.006"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003495\u2010199243060\u201000008"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/18.6.499"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/000456329703400103"},{"key":"e_1_2_10_10_1","unstructured":"AarsandAK Fernandez\u2010CalleP WebsterC et al.The EFLM biological variation database. Accessed August 25 2022.https:\/\/biologicalvariation.eu\/"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/00365519950185229"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/1098\u20102825(2000)14:4<136::AID\u2010JCLA2>3.0.CO;2\u20101"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/80.3.327"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.19.2.196"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.5858\/2002\u2010126\u20100336\u2010ciacbc"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.11648\/j.ijbse.20130102.11"},{"issue":"4","key":"e_1_2_10_17_1","first-page":"286","article-title":"Hyperglycemic macrocytosis in electronically determined mean corpuscular volume. Use of three different automatic cell counters","volume":"15","author":"Planas AT","year":"1985","journal-title":"Ann Clin Lab Sci"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365513.2015.1062534"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2005-916671"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00320301"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.v81.10.2774.bloodjournal81102774"},{"issue":"2","key":"e_1_2_10_22_1","first-page":"59","article-title":"Complete blood count, clinical chemistry, and serology profile by using a single tube of whole blood from mice","volume":"46","author":"Wiedmeyer CE","year":"2007","journal-title":"J Am Assoc Lab Anim Sci"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2001.204"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1177\/0003319717747154"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1649650"},{"issue":"3","key":"e_1_2_10_26_1","first-page":"278","article-title":"Comparison of glucose determinations on blood samples collected in three types of tubes","volume":"43","author":"Li G","year":"2013","journal-title":"Ann Clin Lab Sci"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1055\/s\u20102001\u201016888"}],"container-title":["International Journal of Laboratory Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.14291","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ijlh.14291","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.14291","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T09:03:57Z","timestamp":1773911037000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ijlh.14291"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,2]]},"references-count":26,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2024,10]]}},"alternative-id":["10.1111\/ijlh.14291"],"URL":"https:\/\/doi.org\/10.1111\/ijlh.14291","archive":["Portico"],"relation":{},"ISSN":["1751-5521","1751-553X"],"issn-type":[{"value":"1751-5521","type":"print"},{"value":"1751-553X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,2]]},"assertion":[{"value":"2024-01-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}