{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T12:53:11Z","timestamp":1773924791588,"version":"3.50.1"},"reference-count":15,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T00:00:00Z","timestamp":1714953600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T00:00:00Z","timestamp":1714953600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Leukemic stem cells (LSCs) are the transcriptionally low\/silent cells which are resistant to the tyrosine kinase inhibitor. These have been found to play a pivotal role in disease relapse in chronic myeloid leukemia (CML) cases. The present study evaluated the correlation of absolute CML\u2010LSC count in the peripheral blood (PB) at diagnosis and achievement of major molecular response (MMR) at 12\u2009months in patients of CML\u2010CP.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This was a prospective, observational, non\u2010interventional single center study including newly diagnosed adult (&gt;18\u2009yrs) CML\u2010CP patients. Absolute CD26\u2009+\u2009CML\u2010LSC quantification was done by multiparametric flow cytometry. Patients were treated with Imatinib treatment and subsequently monitored at 3\u2010month intervals for BCR::ABL transcript levels. MMR was defined as a BCR::ABL1 transcript level of less than 0.1% on international scale.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 89 patients were enrolled in the study out of which 40.5% achieved MMR at 12\u2009months. There was a significant difference in the median absolute CML\u2010LSC count of the patients who achieved MMR at 12\u2009months as compared to those who did not (58.5 vs 368.1 cells\/\u03bcL;\n                      <jats:italic>p<\/jats:italic>\n                      value\u2009&lt;0.001). Using a ROC analysis, a count of &lt;165.69 CML LSC\/\u03bcL was identified to have a sensitivity of 83.8% and specificity of 72.4%, in predicting the MMR at 12\u2009months.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Absolute CML\u2010LSC count at diagnosis in the PB predicts the MMR achievement at 12\u2009months. An absolute count of less than 165 cells\/\u03bcL is highly predictive of achieving MMR at 12\u2009months.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ijlh.14301","type":"journal-article","created":{"date-parts":[[2024,5,7]],"date-time":"2024-05-07T02:15:23Z","timestamp":1715048123000},"page":"862-868","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Peripheral blood quantitation of\n                    <scp>CD26<\/scp>\n                    positive leukemic stem cells as a predictor of tyrosine kinase inhibitor response in chronic myeloid leukemia"],"prefix":"10.1111","volume":"46","author":[{"given":"Nitin","family":"Chaudhary","sequence":"first","affiliation":[{"name":"Department of Hematology SGPGIMS  Lucknow 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