{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T12:52:57Z","timestamp":1773924777462,"version":"3.50.1"},"reference-count":20,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,6,5]],"date-time":"2024-06-05T00:00:00Z","timestamp":1717545600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,6,5]],"date-time":"2024-06-05T00:00:00Z","timestamp":1717545600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Accurate quantification of the\n                      <jats:italic>BCR::ABL1<\/jats:italic>\n                      fusion gene in whole blood is pivotal for the clinical management of chronic myeloid leukemia (CML) patients. The fusion protein encoded by\n                      <jats:italic>BCR::ABL1<\/jats:italic>\n                      can vary in size, depending on the\n                      <jats:italic>BCR<\/jats:italic>\n                      and\/or\n                      <jats:italic>ABL1<\/jats:italic>\n                      gene breakpoint. The vast majority of CML patients have a p210\n                      <jats:italic>BCR::ABL1<\/jats:italic>\n                      fusion gene (M\u2010BCR), which can be attributed to the presence of either e14a2 (b3a2) or e13a2 (b2a2) mRNA transcript junctions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Twenty\u2010five CML samples were analyzed in two different ISO15189\u2010accredited centers that both use an Europe Against Cancer\u2010based quantitative polymerase chain reaction (qPCR) protocol. Reanalysis of the sample set with transcript\u2010specific standard curves and digital droplet PCR (ddPCR) were performed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      qPCR quantification revealed a significant (up to 1 log) difference specifically for the e13a2 transcript variant in contrast to e14a2 transcripts (Hodges\u2013Lehman 4.29;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). Reanalysis of the sample set with transcript\u2010specific standard curves abolishes the initial transcript\u2010specific difference (Hodges\u2013Lehman 0.003;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.8192). Comparison of transcript\u2010specific qPCR results of both centers with ddPCR, an absolute quantification method, showed a statically significant association, especially in the lower range, indicating the clinical utility of transcript\u2010specific or absolute quantification methods.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our data show that differences between transcript\u2010specific quantification might exist between centers, leading to potential clinical impact on the follow\u2010up of CML patients. The use of transcript\u2010specific standard curves for qPCR quantification, or absolute quantification, can significantly reduce these differences. Specific attention should be applied to the interpretation of quantification differences of CML patients that switch between diagnostic centers.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ijlh.14320","type":"journal-article","created":{"date-parts":[[2024,6,6]],"date-time":"2024-06-06T02:34:26Z","timestamp":1717641266000},"page":"910-917","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Variant\u2010specific\n                    <i>BCR::ABL1<\/i>\n                    quantification discrepancy in chronic myeloid leukemia"],"prefix":"10.1111","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-5236-8576","authenticated-orcid":false,"given":"Koen","family":"Jacobs","sequence":"first","affiliation":[{"name":"Clinical Laboratory AZ Sint\u2010Lucas Hospital Ghent  Ghent Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alena","family":"Moerman","sequence":"additional","affiliation":[{"name":"Medisch Labo Bruyland Kortrijk  Kortrijk Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0025-5594","authenticated-orcid":false,"given":"Karl","family":"Vandepoele","sequence":"additional","affiliation":[{"name":"Clinical Biology \u2013 Molecular Hematology Ghent University Hospital  Ghent Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tim Van den","family":"Abeele","sequence":"additional","affiliation":[{"name":"Anacura \u2013 Labo Nuytinck Ghent  Ghent Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katrien","family":"De Mulder","sequence":"additional","affiliation":[{"name":"Clinical Laboratory AZ Sint\u2010Lucas Hospital Ghent  Ghent Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Steel","sequence":"additional","affiliation":[{"name":"Hematology AZ ST. Lucas Hospital Ghent  Ghent Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maxim","family":"Clauwaert","sequence":"additional","affiliation":[{"name":"Hematology AZ ST. Lucas Hospital Ghent  Ghent Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henk","family":"Louagie","sequence":"additional","affiliation":[{"name":"Clinical Laboratory AZ Sint\u2010Lucas Hospital Ghent  Ghent Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,6,5]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-020-0776-2"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12556"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9123865"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403135"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-04-150680"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2016.90"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-06-291641"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.25792"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa030513"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.2147\/CMAR.S232752"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejh.13238"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-016-2321-2"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-10-674242"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.24774"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V126.23.597.597"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2013.096537"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2009.009134"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejh.13282"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.18026"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0106250"}],"container-title":["International Journal of Laboratory Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.14320","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ijlh.14320","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.14320","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T09:04:23Z","timestamp":1773911063000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ijlh.14320"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,5]]},"references-count":20,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2024,10]]}},"alternative-id":["10.1111\/ijlh.14320"],"URL":"https:\/\/doi.org\/10.1111\/ijlh.14320","archive":["Portico"],"relation":{},"ISSN":["1751-5521","1751-553X"],"issn-type":[{"value":"1751-5521","type":"print"},{"value":"1751-553X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,5]]},"assertion":[{"value":"2023-10-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}