{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T10:38:40Z","timestamp":1787913520893,"version":"build-2784847793"},"reference-count":17,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,8,5]],"date-time":"2024-08-05T00:00:00Z","timestamp":1722816000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>It is recommended to use two chronometric assays of different principles for the diagnosis of lupus anticoagulant (LA), consisting in diluted Russell Viper Venom Time (dRVVT) and activated Partial Thromboplastin Time (aPTT). Yet, there are only a few integrated aPTT assays; this study aims to evaluate one of them: Cephen LS\/Cephen (Hyphen Biomed).<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>249 samples of patients were included in this study. Normal reference ranges were determined with platelet\u2010poor plasma (PPP) from healthy blood donors. Performances were then evaluated by comparing Cephen LS\/Cephen test results to the results of the laboratory's reference assay for the diagnosis of LA and to clinical data, both on non\u2010anticoagulated and anticoagulated patients' samples (Unfractioned heparin (UFH), Low Molecular Weight Heparin (LMWH), Vitamin K Antagonists (VKA) and apixaban). Interference of UFH, LMWH and VKA were also evaluated thanks to spiking experiment of increasing heparin concentrations or factor deficiency.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Cephen LS\/Cephen test had 48.6% sensitivity towards LA. Although UFH and VKA seemed to interfere with this assay and were likely to cause false negative, LMWH and apixaban did not.<\/jats:p><jats:p>Finally, combination of Cephen LS\/ Cephen with dRVVT had 89.0% sensitivity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Cephen LS\/Cephen seems relevant for LA diagnosis, in combination with dRVVT, and might be used in patients undergoing LMWH or apixaban therapy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.14349","type":"journal-article","created":{"date-parts":[[2024,8,5]],"date-time":"2024-08-05T15:14:07Z","timestamp":1722870847000},"page":"1109-1117","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Evaluation of an integrated activated partial thromboplastin time (Cephen <scp>LS<\/scp>\/Cephen) for the detection of lupus anticoagulant"],"prefix":"10.1111","volume":"46","author":[{"given":"Adrien","family":"Thiriet","sequence":"first","affiliation":[{"name":"Laboratoire d'H\u00e9matologie, H\u00f4pitaux Universitaires de Strasbourg  Strasbourg France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Poindron","sequence":"additional","affiliation":[{"name":"Immunologie Clinique, H\u00f4pitaux Universitaires de Strasbourg  Strasbourg France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Sattler","sequence":"additional","affiliation":[{"name":"Laboratoire d'H\u00e9matologie, H\u00f4pitaux Universitaires de Strasbourg  Strasbourg France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordan","family":"Wimmer","sequence":"additional","affiliation":[{"name":"Laboratoire d'H\u00e9matologie, H\u00f4pitaux Universitaires de Strasbourg  Strasbourg France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delphine","family":"Rolland","sequence":"additional","affiliation":[{"name":"Laboratoire d'H\u00e9matologie, H\u00f4pitaux Universitaires de Strasbourg  Strasbourg France"},{"name":"CNRS UMR7178, Laboratoire de Spectrom\u00e9trie de Masse BioOrganique (LSMBO), Institut Pluridisciplinaire Hubert Curien  Strasbourg France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne\u2010Sophie","family":"Korganow","sequence":"additional","affiliation":[{"name":"Immunologie Clinique, H\u00f4pitaux Universitaires de Strasbourg  Strasbourg France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Mauvieux","sequence":"additional","affiliation":[{"name":"Laboratoire d'H\u00e9matologie, H\u00f4pitaux Universitaires de Strasbourg  Strasbourg France"},{"name":"CNRS UPR3572, Immunologie, Immunopathologie &amp; Chimie Th\u00e9rapeutique (I2CT), Institut de Biologie Mol\u00e9culaire et Cellulaire (IBMC)  Strasbourg France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1040-0086","authenticated-orcid":false,"given":"Agathe","family":"Herb","sequence":"additional","affiliation":[{"name":"Laboratoire d'H\u00e9matologie, H\u00f4pitaux Universitaires de Strasbourg  Strasbourg France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2007.09.001"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538\u20107836.2006.01753.x"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102141.2012.09037.x"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.15047"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2009.09.001"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12921"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.14669"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/rth2.12207"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2006.10.015"},{"issue":"4","key":"e_1_2_10_11_1","first-page":"583","article-title":"Laboratory diagnosis of lupus anticoagulants for patients on oral anticoagulant treatment. Performance of dilute Russell viper venom test and silica clotting time in comparison with Staclot LA","volume":"88","author":"Tripodi A","year":"2002","journal-title":"Thromb Haemost"},{"key":"e_1_2_10_12_1","volume-title":"Anti\u2010Phospholipid Syndrome and Testing for Lupus Anticoagulants: Routine Approach Using Siemens APTT Reagents","author":"Reber G","year":"2013"},{"key":"e_1_2_10_13_1","volume-title":"Cut\u2010off Generation for Percent Correction of Screen by Confirm in Lupus Anticoagulant Testing in Multiple Reagents with a Common Set of Normal Donor Plasmas","author":"Kumano O","year":"2019"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.14846"},{"key":"e_1_2_10_15_1","volume-title":"New dRVVT Assays for Lupus Anticoagulant (LA) Detection with Improved Specificity and Sensitivity: HEMOCLOT LA\u2010S and LA\u2010C","author":"Dunois C","year":"2018"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12640"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.13818"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1055\/s\u20100043\u20101776406"}],"container-title":["International Journal of Laboratory Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.14349","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,15]],"date-time":"2024-11-15T13:06:28Z","timestamp":1731675988000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ijlh.14349"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,5]]},"references-count":17,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["10.1111\/ijlh.14349"],"URL":"https:\/\/doi.org\/10.1111\/ijlh.14349","archive":["Portico"],"relation":{},"ISSN":["1751-5521","1751-553X"],"issn-type":[{"value":"1751-5521","type":"print"},{"value":"1751-553X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,5]]},"assertion":[{"value":"2024-06-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}