{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,24]],"date-time":"2025-07-24T11:23:03Z","timestamp":1753356183388,"version":"3.40.5"},"reference-count":17,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,8,14]],"date-time":"2024-08-14T00:00:00Z","timestamp":1723593600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>We aimed to develop an automated, low\u2010volume method for thrombocyte counting in capillary blood using the Sysmex predilution (PD) mode.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Microsamples were prepared by resuspension of 50\u2009\u03bcL blood in 300\u2009\u03bcL DCL CellPack. Thrombocyte counting was done in the impedance (PLT\u2010I) and fluorescence (PLT\u2010F) channels. The imprecision and bias was evaluated in &gt;394 microsamples from adult blood. Preanalytical factors (skin\u2010piercing, storage, and transportation in our pneumatic tube system) was assessed, and studies on pediatric microsamples were made for comparison. The improvement in analytical quality and turnaround time was examined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>For PLT\u2010F, the imprecision was 1.1%\u20133.7%, and the bias was 10.1% (95% CI: 8.8\u201311.3). After skin\u2010piercing, the bias was 8.1% (95% CI: 5.6\u201310.6) and the imprecision 1.9% (95% CI: 1.3\u20132.5). Thrombocyte counts kept stable after 4\u2009h at room temperature (94.8% [95% CI: 93.2\u201396.4]) and after pneumatic tube transportation [6.7% (95% CI: 4.8\u20138.6)]. The bias of the PD mode for pediatric microsamples was 13.0% (95% CI: \u22128.4\u201334.4) in the PLT\u2010F channel. The automated method had a considerably lower imprecision than the existing manual thrombocyte counting method and reduced turnaround times.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The automated microsample method offers a low\u2010volume alternative for measurement of thrombocytes. The method appears useful also in pediatric samples.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.14354","type":"journal-article","created":{"date-parts":[[2024,8,14]],"date-time":"2024-08-14T11:14:00Z","timestamp":1723634040000},"page":"1044-1051","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["An automated method for thrombocyte counting in capillary microsamples"],"prefix":"10.1111","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4083-7804","authenticated-orcid":false,"given":"Caroline Vasard","family":"Boesen","sequence":"first","affiliation":[{"name":"Department of Clinical Biochemistry Aalborg University Hospital  Aalborg Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vibeke Staun","family":"Christensen","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry Aalborg University Hospital  Aalborg Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus Rosenkilde","family":"Jensen","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry Aalborg University Hospital  Aalborg Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anja Reinert","family":"Hansen","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry Aalborg University Hospital  Aalborg Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1740-7555","authenticated-orcid":false,"given":"Claus Vinter B\u00f8dker","family":"Hviid","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry Aalborg University Hospital  Aalborg Denmark"},{"name":"Department of Clinical Medicine Aalborg University  Aalborg Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,8,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.5546\/aap.2021.eng.e202"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1586\/17474086.2014.902301"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2005-916671"},{"key":"e_1_2_9_5_1","first-page":"11","volume-title":"Estimation of cell number by hemocytometry counting","author":"Michael R","year":"2019"},{"key":"e_1_2_9_6_1","unstructured":"Sysmex Corporation 2017\u20102020 XN series Users Guide. 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