{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:17:15Z","timestamp":1747185435118,"version":"3.40.5"},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,11,6]],"date-time":"2024-11-06T00:00:00Z","timestamp":1730851200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Immune thrombocytopenic purpura (ITP) is an acquired autoimmune disease characterized by reduced platelet counts due to immune system dysregulation caused by many factors, including genetics, autoimmune diseases, infections, and inflammations. Therefore, the current study aimed to evaluate immunological markers such as the expression level of lysosomal associated membrane protein 3 (LAMP\u20103), also known as <jats:bold>CD63<\/jats:bold>, and the expression level of Fc\u2010gamma receptor I (Fc\u03b3RI), also known as <jats:bold>CD64<\/jats:bold> and also investigate the association of Fc\u2010gamma receptor IIIA (<jats:bold>Fc\u03b3RIIIA<\/jats:bold>) 158\u2009V\/F polymorphism to the risk of ITP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 180 subjects; 60 ITP patients, 60 patients with thrombocytopenia of other causes and 60 controls were enrolled into our study. The expression level of CD63 was done using reverse transcription quantitative PCR (RTqPCR), while CD64 expression level was done by flow cytometry. The polymorphism of Fc\u03b3RIIIA 158\u2009V\/F gene was analyzed by polymerase chain reaction followed by restriction fragment length polymorphism (PCR\u2010RFLP) analysis. Finally, CD63 and CD64 protein\u2013protein interactions were done by using the STRING online database.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The expression of CD63 was significantly elevated in ITP patients than thrombocytopenia patients and healthy control. Also there was high expression level of CD64 on granulocytes and monocytes from ITP patients than other groups. Receiver operating characteristic curve (ROC curve) analysis of CD63 showed an area under the curve (AUC) revealed of 1.00, sensitivity of 100% and specificity of 100%; while for CD64 on granulocytes, AUC of 0.998 as well as a sensitivity of 96.66% and specificity of 93.33%. Regarding Fc\u03b3RIIIa 158\u2009V\/F polymorphism, all patients and healthy volunteers included in this study showed the wild FF genotype.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The expression of both CD63 and CD64 were significantly increased in ITP patients and could be good biomarkers to diagnose ITP. Additionally, there is no association between Fc\u03b3RIIIa 158\u2009V\/F polymorphism and the risk of ITP disease.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.14391","type":"journal-article","created":{"date-parts":[[2024,11,6]],"date-time":"2024-11-06T11:34:34Z","timestamp":1730892874000},"page":"255-265","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Diagnostic Implications of <scp>CD63<\/scp> and <scp>CD64<\/scp> Expression Levels and <scp>Fc\u03b3RIIIA<\/scp> 158\u2009V\/F Gene Polymorphism in Primary Immune Thrombocytopenia Adult Patients"],"prefix":"10.1111","volume":"47","author":[{"given":"Dina Samir","family":"Elsaid","sequence":"first","affiliation":[{"name":"Department of Biochemistry Faculty of Science, Ain Shams University  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamer Abd Elhamid","family":"Elbedewy","sequence":"additional","affiliation":[{"name":"Department of 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