{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T04:52:32Z","timestamp":1774932752946,"version":"3.50.1"},"reference-count":16,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,1,12]],"date-time":"2025-01-12T00:00:00Z","timestamp":1736640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFC3402903"],"award-info":[{"award-number":["2023YFC3402903"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Accurate platelet (PLT) counting is crucial for disease diagnosis and treatment, especially under the condition of thrombocytopenia and platelet transfusion. A few PLT counting approaches have been established including impedance and fluorescent methods. The impedance PLT counting (PLT\u2010I) approach could be interfered by small non\u2010PLT particles in the blood, such as RBC\/WBC fragments, microcytes, bacteria, and cryoglobulins. The fluorescent PLT counting methods provide a more accurate but rather costly option. Thus, it is highly desirable to develop a new economic\u2010friendly approach with accurate platelet count. In this study, we introduced a novel hybrid PLT counting (PLT\u2010H) strategy to combine the count of smaller\u2010sized PLTs from impedance channel and larger\u2010sized PLTs from conventional white blood cell (WBC) channel to realize a low\u2010cost PLT count with high accuracy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Blood samples with different PLT levels and no significant interfering factors (confirmed by blood smear) were collected. The PLTs were counted with BC\u20106800Plus (PLT\u2010I) and compared with the PLT counts from the CD41\/CD61 immunoplatelet (immunoPLT) reference method using Beckman Coulter FC\u2010500 flow cytometer. In addition, the morphology and internal structure of PLT treated with hemolytic agents of conventional WBC channel was observed with phase contrast microscopy and electron microscopy. Then the counting accuracy of PLT\u2010H was assessed by comparing PLT count results between BC\u2010720 (PLT\u2010H) and the CD41\/CD61 immunoPLT reference method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>By comparing PLT\u2010I with immunoPLT, it was found that the impedance PLT counting result will not be interfered by small non\u2010PLT particles when the size of PLT smaller than 10\u2009fL. For large PLT (&gt;\u200910\u2009fL), PLT count in conventional WBC channel shows good correlations with immunoPLT. Furthermore, after treated with hemolytic agents the PLT still preserves an intact cellular structure and swells slightly, while RBCs are lysis and disappeared upon hemolytic treatment. Lastly, the novel PLT\u2010H result exhibits a good correlation with immunoPLT with a high correlation factor (<jats:italic>r<\/jats:italic>\u2009=\u20090.9911).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The new PLT counting method PLT\u2010H achieves high accuracy in blood samples with low\u2010cost, and it provide a novel strategy of combining traditional methods for high accurate counting in hematology laboratories.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.14420","type":"journal-article","created":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T02:04:35Z","timestamp":1736733875000},"page":"407-414","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A New Anti\u2010Interfering Platelet Counting Technology Utilizing Conventional Impedance and White Blood Cell Differential Channel"],"prefix":"10.1111","volume":"47","author":[{"given":"Yi","family":"Ye","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine The Third Xiangya Hospital, Central South University  Changsha People's Republic of China"},{"name":"Department of Laboratory Medicine Xiangya School of Medicine, Central South University  Changsha People's Republic of China"},{"name":"Hematology Product Development Department Shenzhen Mindray Bio\u2010Medical Electronics Co. Ltd  Shenzhen People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gengwen","family":"Chen","sequence":"additional","affiliation":[{"name":"Hematology Product Development Department Shenzhen Mindray Bio\u2010Medical Electronics Co. Ltd  Shenzhen People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7471-9007","authenticated-orcid":false,"given":"Zongjun","family":"Liu","sequence":"additional","affiliation":[{"name":"Hematology Product Development Department Shenzhen Mindray Bio\u2010Medical Electronics Co. Ltd  Shenzhen People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8998-2482","authenticated-orcid":false,"given":"Yan","family":"Liu","sequence":"additional","affiliation":[{"name":"Hematology Product Development Department Shenzhen Mindray Bio\u2010Medical Electronics Co. Ltd  Shenzhen People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaosen","family":"Bian","sequence":"additional","affiliation":[{"name":"Hematology Product Development Department Shenzhen Mindray Bio\u2010Medical Electronics Co. Ltd  Shenzhen People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donglan","family":"Yao","sequence":"additional","affiliation":[{"name":"Hematology Product Development Department Shenzhen Mindray Bio\u2010Medical Electronics Co. Ltd  Shenzhen People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keqian","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine The Third Xiangya Hospital, Central South University  Changsha People's Republic of China"},{"name":"Department of Laboratory Medicine Xiangya School of Medicine, Central South University  Changsha People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,1,12]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-339-8_15"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/44.6.678"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-553X.2007.00909.x"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1309\/0GLP-K5NU-N1GD-PR8T"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPUAGGB4URL5XO"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.21037\/atm-21-691"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics12010068"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1309\/W612-MYEP-FA7U-8UYA"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1981.00340070122027"},{"issue":"4","key":"e_1_2_10_11_1","first-page":"393","article-title":"Automated Hematology Analysers and Spurious Counts. 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