{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T12:21:46Z","timestamp":1783167706890,"version":"3.54.6"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T00:00:00Z","timestamp":1739923200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Activated protein C resistance (<jats:styled-content style=\"fixed-case\">APCR<\/jats:styled-content>) represents a risk factor for thrombosis and is usually due to factor V Leiden (<jats:styled-content style=\"fixed-case\">FVL<\/jats:styled-content>). Clinicians may order either test (i.e., <jats:styled-content style=\"fixed-case\">APCR<\/jats:styled-content> or <jats:styled-content style=\"fixed-case\">FVL<\/jats:styled-content>) to help assess \u2018thrombophilia\u2019 in patients who present with thrombosis. <jats:styled-content style=\"fixed-case\">APCR<\/jats:styled-content> testing is usually achieved using clot\u2010based assays, whereas <jats:styled-content style=\"fixed-case\">FVL<\/jats:styled-content> is assessed by genetic testing. There are advantages and disadvantages to either approach.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We report updated findings for APCR testing in our geographic region, in part using recent data from the RCPAQAP, an international external quality assessment (EQA) program, with some 50\u201360 participants for APCR testing over the past decade. Data have been updated to cover the past 13\u2009years (2010\u20132023 inclusive), with four samples assessed each year, but with a primary focus on new data from 2020 to 2023 inclusive. In addition, data for APCR testing over several years from four large tertiary\u2010level hospital laboratories have been assessed following a recent change in instrumentation and haemostasis methods.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>EQA data continue to show variable performance in both numerical values and their interpretation for APCR testing, with certain methods providing more consistently correct findings than others. In addition, participant interpretation of their own numerical values and transcription errors seem problematic. Finally, the change in recent laboratory testing has also evidenced local improvements.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>APCR assays and testing laboratories continue to show variability in performance, with two methods (Pefakit and Staclot) showing the best performance overall. Targeted education may be of benefit, as most of the errors appear to originate from a small proportion of laboratories.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.14447","type":"journal-article","created":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T23:33:56Z","timestamp":1740008036000},"page":"720-729","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Activated Protein C Resistance Testing: An Update From Australasia\/Asia\u2010Pacific"],"prefix":"10.1111","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2103-1661","authenticated-orcid":false,"given":"Emmanuel J.","family":"Favaloro","sequence":"first","affiliation":[{"name":"Department of Haematology, Sydney Centres for Thrombosis and Haemostasis, Institute of Clinical Pathology and Medical Research (ICPMR), NSW Health Pathology Westmead Hospital  Westmead New South Wales Australia"},{"name":"Faculty of Science and Health Charles Sturt University  Wagga Wagga New South Wales Australia"},{"name":"School of Medical Sciences, Faculty of Medicine and Health University of Sydney, Westmead Hospital  Westmead New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandya","family":"Arunachalam","sequence":"additional","affiliation":[{"name":"RCPAQAP Haematology  St Leonards New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elysse","family":"Dean","sequence":"additional","affiliation":[{"name":"RCPAQAP Haematology  St Leonards New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahzuza","family":"Salwa","sequence":"additional","affiliation":[{"name":"RCPAQAP Haematology  St Leonards New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica","family":"Ahuja","sequence":"additional","affiliation":[{"name":"Department of Haematology, NSW Health Pathology St George Hospital  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