{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T11:00:19Z","timestamp":1760785219329,"version":"3.41.2"},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T00:00:00Z","timestamp":1741219200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Limited availability of diagnostic tests in low\u2010resource settings hampers the diagnosis and classification of diffuse large B\u2010cell lymphoma (DLBCL). A study was performed to assess the use of resources for classifying DLBCL in South Africa (SA) using \u2018essential\u2019 and \u2018desirable\u2019 investigations as per published guidelines.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A record review was performed of 74 patients newly diagnosed with DLBCL by tissue biopsy at the National Health Laboratory Service (NHLS) in Johannesburg between 1 January 2019 and 31 December 2022. The immunohistochemistry (IHC) and\/or molecular work\u2010up performed for the primary diagnosis of DLBCL and the associated costs were recorded.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The primary diagnosis of DLBCL was based on 34 (45.9%) nodal and 40 (54.1%) extra\u2010nodal biopsy sections. Overall, 60 (81.1%) were from participants living with human immunodeficiency virus (HIV) infection. \u2018Essential\u2019 IHC for CD3, CD10, CD20, Ki\u201067, BCL\u20102, BCL\u20106, MUM\u20101 and \u2018desirable\u2019 fluorescence in\u00a0situ hybridisation (FISH) for <jats:italic>MYC<\/jats:italic> gene rearrangement were most requested for diagnosis. \u2018Essential\u2019 IHC for c\u2010MYC was not performed because of non\u2010availability of the testing. The \u2018essential\u2019 IHC was diagnostic in 97.3%. \u2018Desirable\u2019 FISH for <jats:italic>MYC<\/jats:italic> rearrangement was done in 56 (79.7%) cases, with additional FISH for <jats:italic>BCL2<\/jats:italic> and <jats:italic>BCL6<\/jats:italic> rearrangement performed in cases positive for <jats:italic>MYC<\/jats:italic> rearrangement. The average cost of diagnosis at the NHLS was half that of the recommended diagnostic testing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The advocated \u2018essential\u2019 investigations, in addition to \u2018desirable\u2019 tests where necessary, enabled the accurate and cost\u2010effective diagnosis of DLBCL in SA and are recommended for other parts of the world with limited resources.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.14453","type":"journal-article","created":{"date-parts":[[2025,3,8]],"date-time":"2025-03-08T13:03:27Z","timestamp":1741439007000},"page":"660-668","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Assessment of the Use of Available Resources for Diagnosing Diffuse Large B\u2010Cell Lymphoma in an <scp>HIV<\/scp>\u2010Prevalent Setting"],"prefix":"10.1111","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-4714-8102","authenticated-orcid":false,"given":"Gaone Abigail","family":"Moalosi","sequence":"first","affiliation":[{"name":"Department of Molecular Medicine and Haematology University of Witwatersrand Medical School and National Health Laboratory Service  Johannesburg Gauteng South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4551-0800","authenticated-orcid":false,"given":"Jenifer","family":"Vaughan","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine and Haematology University of Witwatersrand Medical School and National Health Laboratory Service  Johannesburg Gauteng South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1534-2930","authenticated-orcid":false,"given":"Elise","family":"Schapkaitz","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine and Haematology University of Witwatersrand Medical School and National Health Laboratory Service  Johannesburg Gauteng South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,3,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375\u2010023\u201001962\u20105"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21834"},{"key":"e_1_2_9_4_1","unstructured":"National Health Laboratory Services N \u201cSummary Statistics of Cancer Diagnosed Histologically in 2022 \u201daccessed October 30 2023 https:\/\/www.nicd.ac.za\/wp\u2010content\/uploads\/2024\/04\/NCR_ASR_tables_2022_final.pdf."},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.13885"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0135602"},{"key":"e_1_2_9_7_1","unstructured":"UNAIDS \u201cSouth Africa Factsheet \u201d2023accessed October 30 2024 https:\/\/www.unaids.org\/en\/regionscountries\/countries\/southafrica."},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdv036"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.23391"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13027\u2010019\u20100228\u20107"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.aids.0000433236.55937.cb"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102016\u201001\u2010643569"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2022015851"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pathol.2019.08.013"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102003\u201005\u20101545"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqv028"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102017\u201012\u2010820605"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.5045\/br.2022.2022037"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13027\u2010021\u201000378\u2010z"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/92.15.1240"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.4102\/sajo.v4i0.104"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000000211"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2008.20.5450"},{"issue":"1","key":"e_1_2_9_24_1","article-title":"Cell\u2010of\u2010Origin Identification and Prognostic Correlation in HIV\u2010Associated Diffuse Large B\u2010Cell Lymphomas: Results of an Italian Multicentric Study","volume":"132","author":"Nandikolla A. 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