{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T15:09:28Z","timestamp":1787843368983,"version":"build-2784847793"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T00:00:00Z","timestamp":1743120000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Acute myeloid leukemia (AML) is a malignant hematological disease that has shown an increased prevalence in recent years. Despite advancements in treatment, significant limitations remain. Therefore, more information about AML mechanisms is essential to improve therapeutic strategies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>RT\u2010qPCR, IHC, and western blot were used to analyze SPAG6 expression levels. The association between SPAG6 expression and patient survival was evaluated using LeukemiaDB, GEPIA, and Bloodspot databases. Cell viability was detected by CCK\u20108 assay. Flow cytometry was applied to measure cell apoptosis and cell cycle.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Database analysis revealed elevated SPAG6 expression in AML. Subsequent RT\u2010qPCR confirmed high SPAG6 expression in AML, MDS, MPN, and ALL samples. Clinical data analysis demonstrated a positive correlation between SPAG6 expression and risk stratification in AML patients. Notably, it was found that the overall survival time of AML patients with high SPAG6 expression was shorter than that of patients with low SPAG6 expression. Moreover, SPAG6 knockdown in the AML cell line HL60 promoted apoptosis and arrested the cell cycle in the G1 phase.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Therefore, we believe that SPAG6 may be a pro\u2010tumor factor in AML.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.14457","type":"journal-article","created":{"date-parts":[[2025,3,30]],"date-time":"2025-03-30T16:37:27Z","timestamp":1743352647000},"page":"680-689","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["High Expression of <scp>SPAG6<\/scp> Acts as a Pro\u2010Tumor Factor and Associated With Poor Prognosis in Acute Myeloid Leukemia"],"prefix":"10.1111","volume":"47","author":[{"given":"Jie","family":"Luo","sequence":"first","affiliation":[{"name":"Bishan Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiqiu","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Hematology The First Affiliated Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Zang","sequence":"additional","affiliation":[{"name":"Department of Hematology The First Affiliated Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5496-5287","authenticated-orcid":false,"given":"Lin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Hematology The First Affiliated Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,28]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2016.10"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.26822"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers16030677"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-023-01889-6"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-023-05601-y"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1999.5914"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00354-07"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep25840"},{"issue":"21","key":"e_1_2_12_10_1","first-page":"5096","article-title":"WD40\u2010Repeat Protein 62 Is a JNK\u2010Phosphorylated Spindle Pole Protein Required for Spindle Maintenance and Timely Mitotic Progression","volume":"125","author":"Bogoyevitch M. 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