{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T06:57:46Z","timestamp":1774767466751,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T00:00:00Z","timestamp":1743984000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005010","name":"The Italian Association for Cancer Research","doi-asserted-by":"publisher","award":["grant IG\u201022082 and IG\u201029341"],"award-info":[{"award-number":["grant IG\u201022082 and IG\u201029341"]}],"id":[{"id":"10.13039\/501100005010","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>The majority of patients with essential thrombocythemia (ET) show somatic mutations of <jats:italic>JAK2, CALR<\/jats:italic>, or <jats:italic>MPL<\/jats:italic>. Around 10% of cases lack these mutations (\u201ctriple negative\u201d ET, TN\u2010ET). Additionally, some patients with <jats:italic>bona fide<\/jats:italic> \u201cprimary thrombocytosis\u201d (PT) [i.e., high platelet (PLT)\u2010 count with no apparent underlying causes] do not fulfill the histologic criteria of ET. In this context, Next Generation Sequencing (NGS) can provide evidence of clonality and identify patients with different clinical behaviors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We conducted a retro\u2010prospective analysis of 39 patients with TN\u2010PT and correlated the clinical and pathologic features with the molecular findings.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Bone marrow histopathological features were consistent with ET in 60% of cases. After a mean follow up of 11.1\u2009years, no cases of secondary myelofibrosis nor acute leukemia were observed. We reported 15 thrombotic events (TEs) in 10 (25.6%) patients. Considering mutations with a variant frequency\u2009\u2265\u20095%, 15.4% of patients showed at least one mutation (\u201cNGS\u2010positive\u201d); the remaining had no mutations (\u201cNGS\u2010negative\u201d). NGS status predicted the incidence of TEs: NGS\u2010positive patients experienced a significantly higher rate of TEs compared to NGS\u2010negative patients (66.6% vs. 18.2%, respectively; <jats:italic>p =<\/jats:italic> 0.01).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>NGS status represents an adjunctive risk factor for thrombosis in TN\u2010PT and provides useful clinical information.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.14476","type":"journal-article","created":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T03:38:37Z","timestamp":1743997117000},"page":"869-876","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Next Generation Sequencing Identifies Subgroups of Patients With Triple Negative Primary Thrombocytosis With Different Clinical Thrombotic Outcomes"],"prefix":"10.1111","volume":"47","author":[{"given":"Valentina","family":"Sangiorgio","sequence":"first","affiliation":[{"name":"School of Medicine and Surgery University of Milan\u2010Bicocca  Monza Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federica","family":"Mottadelli","sequence":"additional","affiliation":[{"name":"Tettamanti Center Fondazione IRCCS San Gerardo Dei Tintori  Monza Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabio","family":"Pagni","sequence":"additional","affiliation":[{"name":"School of Medicine and Surgery University of Milan\u2010Bicocca  Monza Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabrizio","family":"Cavalca","sequence":"additional","affiliation":[{"name":"Division of Hematology and Bone Marrow Unit Fondazione IRCCS San Gerardo Dei Tintori  Monza 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