{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T12:04:16Z","timestamp":1784289856341,"version":"3.55.0"},"reference-count":11,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T00:00:00Z","timestamp":1747353600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>The impact of ethylenediaminetetraacetic acid (EDTA) on leukocyte count and morphology has led to the current recommendation that smears made from EDTA blood should be prepared within 4\u2009h of sampling. However, previous studies have only been performed on smears with normal cell counts, and smears made from K<jats:sub>2<\/jats:sub>EDTA blood have not been studied in comparison with smears made from direct finger prick. The aim of this study was to investigate the time limit for performing a morphological assessment on smears from EDTA blood without compromising the results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Blood samples from 37 patients with known or suspected abnormal leukocyte morphology were selected, with smears from direct finger prick used as controls. Smears were prepared from the original EDTA tube every 4 h up to 24\u2009h after sampling. The smears were evaluated for differential count, morphological assessment, and smear quality scoring.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>No significant difference in cell count was observed on smears made up to 12\u2009h after sampling for neutrophils (<jats:italic>p<\/jats:italic>\u2009=\u20090.430), lymphocytes (<jats:italic>p<\/jats:italic>\u2009=\u20090.080), monocytes (<jats:italic>p<\/jats:italic>\u2009=\u20090.948) eosinophils (<jats:italic>p<\/jats:italic>\u2009=\u20090.398), basophils (<jats:italic>p<\/jats:italic>\u2009=\u20090.460), and blast cells (<jats:italic>p<\/jats:italic>\u2009=\u20090.239) compared to smears made by finger prick. However, smears prepared later than 8\u2009h postsampling showed a significant increase in morphological changes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>A manual differential leukocyte count can be reliably performed on smears from EDTA blood within 12\u2009h, whereas a comprehensive morphological assessment requires smears to be prepared within 8\u2009h of sampling.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.14498","type":"journal-article","created":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T06:15:58Z","timestamp":1747376158000},"page":"826-831","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Time\u2010Dependent <scp>EDTA<\/scp> Effect on Leukocyte Differential Count and Morphology Compared to Blood Smear Made by Direct Finger Prick"],"prefix":"10.1111","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-1408-0061","authenticated-orcid":false,"given":"Anna","family":"Irding","sequence":"first","affiliation":[{"name":"Department of Clinical Chemistry Link\u00f6ping University  Link\u00f6ping Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filip","family":"Landgren","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry Link\u00f6ping University  Link\u00f6ping Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"Aardal","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Clinical Sciences Link\u00f6ping University  Link\u00f6ping Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,16]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra043442"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/100.4.371"},{"key":"e_1_2_11_4_1","first-page":"5","volume-title":"Quality Assurance in Haematology","author":"Assendelft O. W.","year":"1988"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02982039"},{"key":"e_1_2_11_6_1","first-page":"211","volume-title":"Quality Control in Haematology","author":"Sacker L. S.","year":"1975"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12170"},{"key":"e_1_2_11_8_1","first-page":"1","article-title":"Blood Film Preparation and Staining Procedures","volume":"6","author":"Houwen B.","year":"2000","journal-title":"Laboratory Hematology"},{"issue":"2","key":"e_1_2_11_9_1","first-page":"89","article-title":"Cell and Platelet Stability in Disodium and Trisodium EDTA","volume":"47","author":"Kennedy J. B.","year":"1981","journal-title":"American Journal of Medical Technology"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12181"},{"key":"e_1_2_11_11_1","unstructured":"Equalis. Expertgruppen f\u00f6r hematologi \u201cRekommendation om standardiserad rutinmetod f\u00f6r morfologisk klassificering och bed\u00f6mning av celler i blodutstryk \u201d2018published April 25 2018 https:\/\/www.equalis.se\/media\/3xfbpsny\/s001_morfologisk\u2010klassificering_3\u20101.pdf."},{"key":"e_1_2_11_12_1","unstructured":"Noklus \u201cAnbefalinger: N\u00e5r bor det lages blodutstryk basert p\u00e5 celletellinger og\/eller flagg fra hematologiinstrumenter? \u201dpublished September 20 2021 https:\/\/www.noklus.no\/media\/qvggqaqe\/anbefalinger\u201029jan19_rev\u20102021.pdf."}],"container-title":["International Journal of Laboratory Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.14498","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T20:07:23Z","timestamp":1757707643000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ijlh.14498"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,16]]},"references-count":11,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["10.1111\/ijlh.14498"],"URL":"https:\/\/doi.org\/10.1111\/ijlh.14498","archive":["Portico"],"relation":{},"ISSN":["1751-5521","1751-553X"],"issn-type":[{"value":"1751-5521","type":"print"},{"value":"1751-553X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,16]]},"assertion":[{"value":"2024-12-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}