{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T02:48:44Z","timestamp":1786416524408,"version":"build-2736575974"},"reference-count":25,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T00:00:00Z","timestamp":1750032000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF\u20102022R1F1A1073963"],"award-info":[{"award-number":["NRF\u20102022R1F1A1073963"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["RS\u20102025\u201000519514"],"award-info":[{"award-number":["RS\u20102025\u201000519514"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>T\u2010cell receptor constant beta chain 1 (TRBC1) has emerged as a potential clonal marker for T\u2010cell lymphoma. This study evaluated TRBC1 expression using flow cytometry in healthy individuals and various clinical specimens, assessing its clinical utility in the diagnosis of T\u2010cell lymphomas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Flow cytometric analysis was performed on peripheral blood specimens from 20 healthy individuals and 186 clinical specimens (116 bone marrow aspirates, 18 peripheral bloods, 52 body fluids) from 177 patients for the exclusion or differential diagnosis of T\u2010cell lymphoma using a panel of nine monoclonal antibodies: CD45, CD3, CD4, CD8, CD56, CD2, CD5, CD7, and TRBC1.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>TRBC1 showed polytypic expression in healthy individuals with a median of 38.6% (range, 33.9%\u201346.1%) in CD4+ T cells and 26.3% (range, 10.9%\u201344.2%) in CD8+ T cells (<jats:italic>p\u2009&lt;<\/jats:italic>\u20090.001). TRBC1 expression in non\u2010T\u2010cell lymphoma specimens was comparable to that in healthy peripheral blood, except for bone marrow, which showed slightly higher TRBC1 expression. All 20 T\u2010cell lymphoma cases exhibited monotypic TRBC1 expression, with a predominance of TRBC1\u2212 (65%). Additionally, T\u2010cell clones of uncertain significance (TCUS) were detected in 12.7% of non\u2010T\u2010cell lymphoma specimens, predominantly in CD8+ T cells and associated with cytopenic and reactive conditions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study demonstrates the clinical utility of flow cytometric TRBC1 assessment in diagnosing T\u2010cell lymphomas, characterizing TRBC1 expression patterns, and identifying TCUS in a large cohort of specimens without T\u2010cell lymphoma involvement across diverse clinical settings. Given the relatively frequent detection of TCUS, these findings underscore the importance of interpreting TRBC1 flow cytometry results within the appropriate clinical context.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.14508","type":"journal-article","created":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T08:31:04Z","timestamp":1750149064000},"page":"840-848","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Flow Cytometric Assessment of <scp>TRBC1<\/scp> Expression for the Diagnosis of T\u2010Cell Lymphoma: Clinical Utility and Pitfalls Related to T\u2010Cell Clones of Uncertain Significance"],"prefix":"10.1111","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-5827-9680","authenticated-orcid":false,"given":"SooHo","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Genetics Samsung Medical Center, Sungkyunkwan University School of Medicine  Seoul 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Sungkyunkwan University School of Medicine  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0553-7096","authenticated-orcid":false,"given":"Hyun\u2010Young","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Genetics Samsung Medical Center, Sungkyunkwan University School of Medicine  Seoul 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