{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T13:46:01Z","timestamp":1783172761166,"version":"3.54.6"},"reference-count":22,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T00:00:00Z","timestamp":1750636800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009776","name":"McMaster University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009776","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Light transmission platelet aggregometry (LTA) is useful to diagnose platelet function disorders (PFD). We evaluated the precision and reproducibility of LTA with low platelet count platelet\u2010rich plasma (LPRP).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>LPRP maximal aggregation (MA) precision for informative agonists and LTA reproducibility were assessed using multiple replicates for adjusted control LPRP (CLPRP) and retrospective analysis of several decades of consecutive patient LPRP (PLPRP) and CLPRP tests with replicates between and\/or within tests.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Intra\u2010subject CLPRP had acceptable MA CVs and tracings unless platelets were \u2264\u200925\u2009\u00d7\u200910\n                      <jats:sup>9<\/jats:sup>\n                      \/L. Among tests with replicates, PLPRP with \u2264\u200925\u2009\u00d7\u200910\n                      <jats:sup>9<\/jats:sup>\n                      platelets\/L were uncommon (6\/247 samples) and 5\/6 showed pathognomonic Bernard\u2013Soulier syndrome (BSS) findings. Among evaluated patients (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u2009195; diverse diagnoses), 44.6% had abnormal LTA findings on \u2265\u20091 tests after excluding single, within\u2010test MA outliers in 21\/194 PLPRP and 2\/27 CLPRP tests. Median, intra\u2010subject, within\u2010test coefficients of variation (CVs) for PLPRP and CLPRP MA responses to informative agonists were acceptable for most agonists, with higher CV for 0.5\u2009mg\/mL ristocetin, weak agonists, and impaired responses, and only small differences between MA estimates across agonists. Between\u2010test agreement was good for MA responses, with 80.5% of patients and 98.1% of controls having consistent overall LTA findings, with significantly more patients than controls having consistently abnormal LTA findings (46.3% vs. 0%;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Diagnostic LTA with LPRP has acceptable precision and reproducibility when evaluated with informative agonists. Nonetheless, caution is warranted when testing samples with \u2264\u200925\u2009\u00d7\u200910\n                      <jats:sup>9<\/jats:sup>\n                      platelets\/L, which often show BSS findings.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ijlh.14518","type":"journal-article","created":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T19:09:18Z","timestamp":1750705758000},"page":"1147-1157","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["How Trustworthy is Light Transmittance Platelet Aggregometry With Low Platelet Count Samples? Insights From Test Replicates and Retrospective Analysis of Several Decades of Diagnostic Samples"],"prefix":"10.1111","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2843-0817","authenticated-orcid":false,"given":"Catherine P. M.","family":"Hayward","sequence":"first","affiliation":[{"name":"Department of Pathology and Molecular Medicine McMaster University  Hamilton Canada"},{"name":"Department of Medicine McMaster University  Hamilton Canada"},{"name":"Special Coagulation Laboratory Hamilton Regional Laboratory Medicine Program  Hamilton Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rabab","family":"Al Dawood","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine McMaster University  Hamilton Canada"},{"name":"Department of Pathology and Laboratory Medicine King Fahad Specialist Hospital  Dammam Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lindsay","family":"Wice","sequence":"additional","affiliation":[{"name":"Special Coagulation Laboratory Hamilton Regional Laboratory Medicine Program  Hamilton Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6431-5525","authenticated-orcid":false,"given":"Karen A.","family":"Moffat","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine McMaster University  Hamilton Canada"},{"name":"Department of Medicine McMaster University  Hamilton Canada"},{"name":"Special Coagulation Laboratory Hamilton Regional Laboratory Medicine Program  Hamilton Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,23]]},"reference":[{"issue":"1","key":"e_1_2_9_2_1","first-page":"134","article-title":"An Evaluation of Methods for Determining Reference Intervals for Light Transmission Platelet Aggregation Tests on Samples With Normal or Reduced Platelet Counts","volume":"100","author":"Hayward C. 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