{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T15:37:39Z","timestamp":1777563459055,"version":"3.51.4"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T00:00:00Z","timestamp":1756166400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>The European LeukemiaNet (ELN) 2022 classification introduced significant modifications to acute myeloid leukemia (AML) categorization, including refined criteria for AML with myelodysplasia\u2010related cytogenetic abnormalities (AML\u2010MRC). While cytogenetic analysis is essential for a definitive diagnosis, the question remains whether flow cytometry can aid in the initial identification of this AML subgroup. This study aimed to characterize the immunophenotypic profiles of AML\u2010MRC and validate previously reported immunophenotypic patterns of AML with t(8;21) and inv(16) using flow cytometry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This retrospective study analyzed 911 non\u2010acute promyelocytic leukemia (APL) AML cases. Flow cytometric immunophenotyping was performed using a comprehensive panel of 23 markers. Statistical analysis included univariate and multivariate logistic regression to identify discriminatory markers.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Among 911 patients, 241 (26.5%) were classified as AML\u2010MRC. AML\u2010MRC patients were significantly older (mean age: 55.9 vs. 47.9\u2009years,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001) and presented with lower WBC counts (median: 8.9 vs. 24.2\u2009\u00d7\u200910^9\/L,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001) compared to non\u2010MRC cases. AML\u2010MRC demonstrated higher expression of CD34 (75.9% vs. 57.6%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), CD41a (10.8% vs. 4.5%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.002) and CD235a (5.8% vs. 1.2%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), with CD235a showing the highest discriminatory power (OR 4.458, 95% CI 1.720\u201311.552). For core\u2010binding factor AML, AML with t(8;21) exhibited characteristic expression of CD19 (46.3% vs. 9.4%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001) and CD56 (72.5% vs. 34.5%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), while AML with inv(16) showed distinctive CD34 (88.9% vs. 61.7%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.004) and CD14 (59.3% vs. 18.1%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001) expression patterns.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study identifies markers that distinguish AML\u2010MRC, including CD235a, CD41a, and CD34. This suggests that acute erythroid leukemia and acute megakaryocytic leukemia are subsets within the AML\u2010MRC category. Additionally, the study validates previously reported immunophenotypic characteristics of AML with t(8;21) and inv(16).<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ijlh.14546","type":"journal-article","created":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T07:44:09Z","timestamp":1756194249000},"page":"1099-1106","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Can Flow Cytometry Immunophenotyping Predict Cytogenetic Abnormalities in Acute Myeloid Leukemia? A Focus on Myelodysplasia\u2010Related Cytogenetic Abnormalities"],"prefix":"10.1111","volume":"47","author":[{"given":"Orathai","family":"Promsuwicha","sequence":"first","affiliation":[{"name":"Division of Hematology, Department of Medicine Faculty of Medicine Siriraj Hospital, Mahidol University  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weerapat","family":"Owattanapanich","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Medicine Faculty of Medicine Siriraj Hospital, Mahidol University  Bangkok Thailand"},{"name":"Center of Excellence of Siriraj Adult Acute Myeloid\/Lymphoblastic Leukemia Faculty of Medicine Siriraj Hospital, Mahidol University  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Supattra","family":"Kankhaw","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Medicine Faculty of Medicine Siriraj Hospital, Mahidol University  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5717-515X","authenticated-orcid":false,"given":"Theera","family":"Ruchutrakool","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Medicine Faculty of Medicine Siriraj Hospital, Mahidol University  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Smith","family":"Kungwankiattichai","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Medicine Faculty of Medicine Siriraj Hospital, Mahidol University  Bangkok Thailand"},{"name":"Center of Excellence of Siriraj Adult Acute Myeloid\/Lymphoblastic Leukemia Faculty of Medicine Siriraj Hospital, Mahidol University  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,8,26]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2022016867"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqz206"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.25769"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V80.12.3182.3182"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V80.2.470.470"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u20102141.1998.00796.x"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/1042819031000116698"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20104939\u20109650\u20106_15"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2010.312"},{"issue":"10","key":"e_1_2_13_11_1","first-page":"1783","article-title":"Proposals for the Immunological Classification of Acute Leukemias. 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