{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T16:18:37Z","timestamp":1781885917723,"version":"3.54.5"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T00:00:00Z","timestamp":1756080000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T00:00:00Z","timestamp":1756080000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Immunoassays detecting anti\u2010PF4\/H antibodies must be sensitive to exclude heparin\u2010induced thrombocytopenia (HIT), and optical density (OD) values are useful for confirming HIT, but no calibration is currently available.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To study the impact of OD values on the performance of the Asserachrom HPIA IgG in a cohort of patients with suspected HIT, and the value of a calibration performed with 5B9, a HIT monoclonal antibody.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The HPIA IgG was performed in 170 patients with a high or intermediate probability of HIT. Results were expressed in OD\n                      <jats:sub>450<\/jats:sub>\n                      or \u20185B9 equivalent\u2019 units, using a calibration done with 5B9. HIT was confirmed when HPIA and SRA\/PF4 tests were positive.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>HIT was excluded in 97 cases because HPIA and SRA\/PF4 were negative. The HPIA was positive in 73 cases and HIT confirmed in 43 cases (SRA\/PF4+). Applying an OD threshold of 1.05, the NPV and PPV of the test were 98% and 83%, respectively. Calibration of HPIA with 5B9 did not improve its performance, since similar AUC values (ROC curves) were obtained whether results were expressed in OD values or in equivalent units of 5B9. Bayesian analysis showed that in patients with an intermediate pre\u2010test probability of HIT, the post\u2010test probability equalled 1% when OD was less than 1, and 100% when OD was over 2.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>5B9 as a calibrator failed to improve the performance of HPIA, but this assay can reliably exclude (when negative) or confirm HIT (when OD\u2009&gt;\u20092), without requiring a functional assay.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ijlh.14547","type":"journal-article","created":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T02:15:32Z","timestamp":1756174532000},"page":"165-171","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of 5B9 as a Calibrator or Expression of Results in Absorbance Values for the Diagnosis of Hit With a PF4\/Heparin Specific Elisa"],"prefix":"10.1111","volume":"48","author":[{"given":"Claire","family":"Pouplard","sequence":"first","affiliation":[{"name":"Laboratory of Haematology\u2010Hemostasis University Hospital of Tours \u2013 INSERM UMR 1327 ISCHEMIA  Tours France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"No\u00e9mie","family":"Charuel","sequence":"additional","affiliation":[{"name":"Laboratory of Haematology\u2010Hemostasis University Hospital of Tours \u2013 INSERM UMR 1327 ISCHEMIA  Tours France"},{"name":"Stago  Asni\u00e8res sur Seine Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estelle","family":"Archer","sequence":"additional","affiliation":[{"name":"Laboratory of Haematology\u2010Hemostasis University Hospital of Tours \u2013 INSERM UMR 1327 ISCHEMIA  Tours France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Vayne","sequence":"additional","affiliation":[{"name":"Laboratory of 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