{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T21:18:33Z","timestamp":1769894313462,"version":"3.49.0"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T00:00:00Z","timestamp":1756857600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T00:00:00Z","timestamp":1756857600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["I\u20101145"],"award-info":[{"award-number":["I\u20101145"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009104","name":"Department of Health Research, India","doi-asserted-by":"publisher","award":["DHR\u20101311"],"award-info":[{"award-number":["DHR\u20101311"]}],"id":[{"id":"10.13039\/501100009104","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>B\u2010cell acute lymphoblastic leukemia (B\u2010ALL) is genetically heterogeneous. We assessed the utility of FusionPlex ALL targeted RNA sequencing panel in detecting gene fusions and other genomic lesions in B\u2010ALL.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The high\u2010risk B\u2010ALL, negative for common recurrent gene fusions (RGF), that is,\n                      <jats:italic>BCR::ABL1<\/jats:italic>\n                      ,\n                      <jats:italic>ETV6::RUNX1<\/jats:italic>\n                      ,\n                      <jats:italic>TCF3::PBX1<\/jats:italic>\n                      and\n                      <jats:italic>KMT2A::AFF1<\/jats:italic>\n                      , were analysed with RNA\u2010based targeted sequencing 81\u2010gene\u2010panel FusionPlex ALL (IDT, USA). Multiplex ligation\u2010dependent probe amplification (MLPA) was used for\n                      <jats:italic>IKZF1<\/jats:italic>\n                      deletions and flow\u2010cytometry for\n                      <jats:italic>CRLF2<\/jats:italic>\n                      expression and ploidy analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Out of 32 samples, 27 were high\u2010risk B\u2010ALL cases (median age 16 (1\u201341) years) and 5 B\u2010ALL controls with known fusions for validation. The fusions were detected in 6\/27 (22%) RGF\u2010negative B\u2010ALL cases; 2 with\n                      <jats:italic>EPOR::IGH<\/jats:italic>\n                      and 1 each\n                      <jats:italic>P2RY8::IGH, PAX5::ETV6, SNX2::ABL1, IKZF1::CIITA<\/jats:italic>\n                      . In addition,\n                      <jats:italic>IKZF1<\/jats:italic>\n                      and\/or\n                      <jats:italic>PAX5<\/jats:italic>\n                      gene deletions resulting in the formation of oncogenic\/novel isoforms were detected in 75% (15\/20) samples positive on MLPA. Flow\u2010cytometry\n                      <jats:italic>CRLF2<\/jats:italic>\n                      overexpression was noted in 60% (9\/15) tested samples which correlated well with targeted RNAseq\n                      <jats:italic>CRLF2<\/jats:italic>\n                      gene expression.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The targeted sequencing approach can help in detecting known and novel fusions in B\u2010ALL, novel breakpoints in the known fusions, gene deletions as oncogenic\/novel isoforms and\n                      <jats:italic>CRLF2<\/jats:italic>\n                      expression.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ijlh.14551","type":"journal-article","created":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T11:06:07Z","timestamp":1756897567000},"page":"117-129","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Application of Targeted RNA\u2010Sequencing in High\u2010Risk B\u2010Cell Acute Lymphoblastic Leukemia (B\u2010ALL): Identifying Fusions,\n                    <i>IKZF1<\/i>\n                    Deletions, and\n                    <i>CRLF2<\/i>\n                    Expression in an Indian Cohort"],"prefix":"10.1111","volume":"48","author":[{"given":"Sanjeev Kumar","family":"Gupta","sequence":"first","affiliation":[{"name":"Laboratory Oncology Unit Dr BRA IRCH, All India Institute of Medical Sciences (AIIMS)  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9141-0299","authenticated-orcid":false,"given":"Gadha 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