{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T19:22:16Z","timestamp":1772997736866,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T00:00:00Z","timestamp":1762732800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T00:00:00Z","timestamp":1762732800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Numerous algorithms based on recent developments of additional parameters on last generation haematology analysers have opened new perspectives for hereditary spherocytosis (HS) screening. Although some studies have demonstrated significant age\u2010related variation for reticulocyte\u2010derived parameters, these algorithms did not consider patients' age. Moreover, the apparent lack of short\u2010term stability of some parameters could represent an obstacle to their use if not performed as first\u2010line testing. This study aimed to identify robust and discriminating parameters that could be used for HS screening at any patients' age.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>After an evaluation of the reference values and stability of the additional parameters offered by the Sysmex XN\u20109000, we retrospectively collected and analysed the haematological parameters of 103 confirmed HS patients and 124 controls. The HS group was also compared to 116 samples from patients affected with other red blood cell or iron disorders.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Our findings confirm that the reticulocyte count (Ret) and its ratio to the immature reticulocyte fraction (Ret\/IRF) are the most effective and robust parameters for HS screening, with stability up to 48 and 96\u2009h at room temperature (RT) and 4\u00b0C, respectively. The Ret\/IRF ratio was mostly affected by patients' age rather than haemoglobin levels. We therefore proposed different algorithms that can be adapted to the laboratory's needs and that incorporate age\u2010specific thresholds for Ret\/IRF. The combination of Ret and Ret\/IRF allowed a sensitivity between 91.3% and 100% and a specificity between 77.1% and 94.2%.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This new age\u2010dependent algorithm using Sysmex's most stable parameters offers an easy\u2010to\u2010use tool for HS screening, even in cases of delayed or residual blood analysis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ijlh.70023","type":"journal-article","created":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T02:03:16Z","timestamp":1762826596000},"page":"316-326","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Role of Additional Erythrocyte and Reticulocyte Parameters Offered by Sysmex\n                    <scp>XN<\/scp>\n                    \u20109000 in the Diagnostic Workup of Hereditary Spherocytosis: A New Screening Algorithm According to Age"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4512-7588","authenticated-orcid":false,"given":"Anne\u2010Sophie","family":"Adam","sequence":"first","affiliation":[{"name":"Department of Clinical Chemistry Laboratoire Hospitalier Universitaire de Bruxelles (LHUB\u2010ULB), Universit\u00e9 Libre de Bruxelles  Brussels Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Cotton","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry Laboratoire Hospitalier Universitaire de Bruxelles (LHUB\u2010ULB), Universit\u00e9 Libre de Bruxelles  Brussels Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1628-4309","authenticated-orcid":false,"given":"Danai","family":"Poutakidou","sequence":"additional","affiliation":[{"name":"Department of Haematology Laboratoire Hospitalier Universitaire de Bruxelles (LHUB\u2010ULB), Universit\u00e9 Libre de Bruxelles  Brussels Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B\u00e9atrice","family":"Gulbis","sequence":"additional","affiliation":[{"name":"Human Genetic Centre, H\u00f4pital Erasme, Universit\u00e9 Libre de Bruxelles  Brussels Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,10]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12657"},{"key":"e_1_2_10_3_1","first-page":"274","article-title":"Hereditary Red Cell Membrane Defects: Diagnostic and Clinical Aspects","volume":"9","author":"Barcellini W.","year":"2011","journal-title":"Blood 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