{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T07:49:41Z","timestamp":1793346581169,"version":"4.5.0"},"reference-count":7,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T00:00:00Z","timestamp":1763942400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T00:00:00Z","timestamp":1763942400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2026,4]]},"abstract":"ABSTRACT<\/jats:title>\n \n Objectives<\/jats:title>\n The all\u2010trans\u2010retinoic acid (ATRA) treatment used in acute promyelocytic leukemia (APL) has the particularity of inducing differentiation of the leukemia cells. As a result, within the same smear, several stages of cellular differentiation of the granular lineage are present, which can make it difficult to distinguish elements when counting cells.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n The aim of this study is to use the technical data from Sysmex haematology equipment in patients with APL to improve their cytological monitoring and harmonize blood count results from one operator to another. We collected 132 samples from patients with APL over a period of 45\u2009days from the day of diagnosis and divided them into two groups: presence or absence of blast cells on the blood smear.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n Seven parameters of leukocyte subpopulations were selected for cytological monitoring. We developed a decision algorithm based on these, including decision thresholds for predicting the presence or absence of blasts. These data were verified on a validation cohort of 110 samples with APL, set up at a different site from the training cohort. The algorithm predicted the presence of blast cells in these samples with a sensitivity of 84.6% and a specificity of 86.7%.<\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n In the context of cytological monitoring of patients with APL, the study of technical data has not been developed yet, despite the fact that this is a difficult and sometimes nonhomogeneous task from one operator to another. The proposed algorithm could be a facilitating tool in cytology to harmonize practices both within and between sites.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/ijlh.70031","type":"journal-article","created":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T23:49:56Z","timestamp":1764028196000},"page":"345-352","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Contribution of\n XN<\/scp>\n \u201010 Sysmex Parameters in the Cytological Monitoring of Acute Promyelocytic Leukemia"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-5573-7926","authenticated-orcid":false,"given":"Lucille","family":"De Maria","sequence":"first","affiliation":[{"name":"Centre Hospitalier Universitaire de Nice Nice France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Logan","family":"Baldini","sequence":"additional","affiliation":[{"name":"Centre Antoine Lacassagne Nice Nice France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-6461-7488","authenticated-orcid":false,"given":"Joy","family":"Mouanes Abelin","sequence":"additional","affiliation":[{"name":"Centre Hospitalier Universitaire de Nice Nice France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corinne","family":"Ferrero","sequence":"additional","affiliation":[{"name":"Centre Hospitalier Universitaire de Nice Nice France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yann","family":"Boursier","sequence":"additional","affiliation":[{"name":"Centre Hospitalier Universitaire de Nice Nice France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yael","family":"Berda\u2010Haddad","sequence":"additional","affiliation":[{"name":"Assistance publique des h\u00f4pitaux de Marseille Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Toulon","sequence":"additional","affiliation":[{"name":"Centre Hospitalier Universitaire de Nice Nice France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,24]]},"reference":[{"key":"e_1_2_12_2_1","unstructured":"Sant\u00e9 Publique France \u201cEstimations nationales de l'incidence et de la mortalit\u00e9 par cancer en France m\u00e9tropolitaine entre 1990 et 2018 volume 2 \u2010 H\u00e9mopathies malignes \u201d2019."},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12030648"},{"key":"e_1_2_12_4_1","doi-asserted-by":"crossref","unstructured":"\u201cTissue Transglutaminase Contributes to the All\u2010Trans\u2010Retinoic Acid\u2013Induced Differentiation Syndrome Phenotype in the NB4 Model of Acute Promyelocytic Leukemia \u201dBlood American Society of Hematology accessed May 13 2024 https:\/\/ashpublications.org\/blood\/article\/116\/19\/3933\/28053\/Tissue\u2010transglutaminase\u2010contributes\u2010to\u2010the\u2010all.","DOI":"10.1182\/blood-2010-01-266064"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.3343\/alm.2022.42.4.398"},{"key":"e_1_2_12_6_1","unstructured":"\u201cH20\u2010A2: Reference Leukocyte (WBC) Differential Count (Proportional) and Evaluation of Instrumental Methods; Approved Standard\u2014Second Edition\u201d."},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12288\u2010021\u201001488\u20109"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.3343\/alm.2016.36.6.607"}],"container-title":["International Journal of Laboratory Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.70031","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ijlh.70031","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.70031","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T16:33:42Z","timestamp":1772987622000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ijlh.70031"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,24]]},"references-count":7,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1111\/ijlh.70031"],"URL":"https:\/\/doi.org\/10.1111\/ijlh.70031","archive":["Portico"],"relation":{},"ISSN":["1751-5521","1751-553X"],"issn-type":[{"value":"1751-5521","type":"print"},{"value":"1751-553X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,24]]},"assertion":[{"value":"2025-07-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}