{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T15:13:48Z","timestamp":1778253228721,"version":"3.51.4"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T00:00:00Z","timestamp":1766966400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T00:00:00Z","timestamp":1766966400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Minimal\/measurable residual disease (MRD) is a key prognostic factor in B\u2010lymphoblastic leukemia\/lymphoma (B\u2010ALL\/LBL), commonly assessed via multiparametric flow cytometry (MFC). This study evaluated 12 leukemia\u2010associated immunophenotype (LAIP) markers\u2014CD81, CD86, CD123, CD58, CD9, CD73, CD13\/CD33, CD44, CD97, CD66c, CD49f, and CD304\u2014to determine their suitability for MRD detection.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Marker expression was analyzed in 103 B\u2010ALL cases at diagnosis, and 54 post\u2010therapy follow\u2010up samples, including 16 MRD\u2010positive cases. Additionally, 25 non\u2010B\u2010ALL bone marrow samples were examined for marker expression in hematogones. Clinical and genetic correlation was also performed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Median age of patients was 9\u2009years (range: 0.3\u201389), with male: female ratio of 1:1. We found that CD97, CD73, CD86, and CD58 are excellent markers for MRD analysis in B\u2010ALL, as they are expressed in more than 85% of cases at baseline, and their expression is preserved in more than 75% of cases post\u2010therapy. Two other extremely promising markers are CD81 and CD49f, both of which are expressed as LAIP in more than 50% of B\u2010ALL cases, with retention of expression in more than 85% of cases post\u2010therapy, and importantly, expression as LAIP in some post\u2010therapy samples despite their absence at baseline. Hyperdiploidy was significantly associated with expression of CD86, CD97, CD123, CD66c, and CD9;\n                      <jats:italic>BCR<\/jats:italic>\n                      ::\n                      <jats:italic>ABL1<\/jats:italic>\n                      fusion was associated with CD49f, CD81, and CD66c.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>CD97, CD73, CD86, and CD58 are the best amongst newer markers in B\u2010ALL MRD assessment. Our findings support integrating these into MRD panels to enhance post\u2010therapy MRD detection, thus improving prognostication and guiding treatment decisions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ijlh.70053","type":"journal-article","created":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T07:59:37Z","timestamp":1767081577000},"page":"589-597","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative Analysis of 12 Flow Cytometry\u2010Based Markers in B\u2010Lymphoblastic Leukemia\/Lymphoma and Their Utility in Detecting Minimal\/Measurable Residual Disease"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4235-0805","authenticated-orcid":false,"given":"Tharageswari","family":"Srinivasan","sequence":"first","affiliation":[{"name":"Department of Hematology Postgraduate Institute of Medical Education and Research  Chandigarh 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