{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T04:05:02Z","timestamp":1783915502672,"version":"3.55.0"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T00:00:00Z","timestamp":1776384000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T00:00:00Z","timestamp":1776384000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC2406300"],"award-info":[{"award-number":["2022YFC2406300"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>The bone marrow microenvironment plays crucial roles in the pathogenesis of multiple myeloma. Previous studies have shown that there are differences in the metabolomics of bone marrow suspension and plasma, but there is rare research on bone marrow biopsy tissue. This study aims to establish a new metabolomics approach to analyze bone marrow biopsy tissues and explore its diagnostic value.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We collected bone marrow biopsy tissues from 19 newly\u2010diagnosed multiple myeloma(MM), 29 monoclonal gammaglobulinemia of undetermined significance(MGUS) and 30 lymphoma without bone marrow invasion as the controls. Metabolomics analysis was conducted using high\u2010performance liquid chromatography\u2010mass spectrometry. Diagnostic Biomarkers and associated pathway were analyzed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The metabolic profiles differed between the controls and MGUS or MM. For MGUS versus MM comparison, Phenylalanyl\u2010Valine and N\u2010Acetylornithine were significantly down\u2010regulated in the MM while N\u2010Acetylornithine has the best diagnostic value with the sensitivity and specificity of 87.5% and 71.4%. For the controls vs. MM comparison, N\u2010Acetylornithine and Aspartyl\u2010Arginine differed markedly. Compared with the controls group, N\u2010Acetylornithine was significantly down\u2010regulated in the MM group, and the AUC of N\u2010Acetylornithine for MM was 0.933, with sensitivity and specificity of (90.0% and 93.3%). N\u2010Acetylornithine displayed the same trends as the comparison between MGUS and MM. Furthermore, when comparing MGUS and MM, Arginine biosynthesis was identified as significantly disrupted in which N\u2010Acetylornithine was involved.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We establish a novel method for metabolomic profiling of bone marrow biopsy tissue and provide evidence of metabolic alterations, particularly reduced N\u2010acetylornithine levels, in MGUS and MM patients.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ijlh.70116","type":"journal-article","created":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T05:09:15Z","timestamp":1776488955000},"page":"839-847","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["N\u2010Acetylornithine Depletion in Bone Marrow Biopsy: A Novel Microenvironment\u2010Specific Hallmark for Multiple Myeloma 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